LOCUS       sequence-286674-        2345 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3377
FEATURES             Location/Qualifiers
     source          1..2345
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2177..2201)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2209..2250
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgggcatgag gatcaaactg
       61 caaagcacca accaccccaa caacctgctg aaggaactca acaagtgccg gctctcagag
      121 accatgtgcg acgtcaccat tgtggtgggg agccgctcct tcccggccca caaggctgtg
      181 ctggcctgtg cagctggcta cttccagaac ctcttcctga atactgggct tgatgctgcc
      241 aggacctatg tggtggactt catcacccct gccaactttg agaaggttct gagctttgtc
      301 tacacttcag aactcttcac agacctgatc aatgttgggg tcatctacga ggtagctgag
      361 cgtctgggta tggaggacct cctccaggcc tgtcactcta cctttcctga tctggagagc
      421 actgccaggg ccaagcccct gaccagcacc agtgagagcc actctggtac cctgagttgt
      481 ccttcggcag aacctgccca tccccttgga gaactccgag gtggtgggga ctaccttggt
      541 gctgatagaa actatgtgtt gcccagtgat gctggaggga gctataaaga ggaagagaag
      601 aatgttgcca gtgacgctaa ccatagcctg catctgccgc aaccgccccc accaccgcca
      661 aagacagaag accatgacac ccctgctccc ttcacgtcca ttcctagcat gatgacccag
      721 ccactcctag gcactgtcag cacgggcatc cagaccagca cgagctcctg ccagccatac
      781 aaagttcaaa gcaatggaga cttcagtaaa aacagcttcc tcacccctga caatgcagta
      841 gacattacca ctgggaccaa ctcctgtctg agcaatagtg agcactccaa agatcctggc
      901 tttgggcaga tggatgagct ccagctcgag gacctggggg atgatgactt gcagtttgaa
      961 gaccctgctg aggatatagg cacaactgag gaggtgattg agctgagtga tgacagtgag
     1021 gatgagttgg cttttggaga gaatgacaat cgggagaata aggccatgcc ctgccaggtg
     1081 tgcaagaaag ttctagagcc caacattcaa ctgatccggc agcatgctcg ggaccatgtg
     1141 gacctgctga cgggcaactg caaggtctgc gagacccact tccaggaccg aaactcccgg
     1201 gtaactcatg tcctgtccca cattggtatt ttccttttct cctgcgacat gtgtgaaact
     1261 aagttcttta cccagtggca gctgaccctt caccgacggg atggaatatt tgagaacaac
     1321 atcattgtcc accccaacga tcccctgcca gggaagctgg gtctcttttc aggggcagcc
     1381 tccccagagc tgaaatgcgc tgcctgtggg aaagtattgg ccaaagattt ccatgtggtc
     1441 cggggccaca tccttgacca tctaaacttg aagggccagg cctgcagtgt ctgcgaccag
     1501 cgtcacctta acctctgcag cctcatgtgg cacacgctgt cccatctcgg catctcagtc
     1561 ttctcctgtt ctgtctgtgc gaacagcttt gtggactggc atcttctaga gaagcacatg
     1621 gctgtgcacc aaagtctgga agacgccctc ttccactgcc gcttgtgcag ccagagcttc
     1681 aagtcagagg ctgcctatcg ctaccacgtc agccagcaca aatgcaacag tggccttgat
     1741 gcacggcctg gttttgggct gcagcaccca gctctccaga agcggaagct gccagcagag
     1801 gagtttctgg gtgaagagct ggcgctgcag ggccaacctg ggaacagcaa gtatagctgc
     1861 aaggtctgtg gcaaaagatt tgcccacaca agcgaattca actaccaccg gcggatccac
     1921 acgggggaga agccatacca atgtaaggtg tgccacaagt tctttcgagg ccgctcgacc
     1981 atcaagtgcc acctaaagac acactcgggg gccctcatgt accgctgcac agtctgtggg
     2041 cactacagtt ccacccttaa cctcatgagc aaacatgttg gtgtgcacaa aggcagcctc
     2101 ccccctgact tcaccatcga gcagaccttc atgtacatca tccattccaa agaggcggat
     2161 aagaacccgg acagtttgcc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     2221 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     2281 atttcgattt cttggcttta tatatcttgt ggaaaggacg aacccaaagc tgtcgtgggg
     2341 gaccg
//
