LOCUS       sequence-286677-        1625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3378
FEATURES             Location/Qualifiers
     source          1..1625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1457..1481)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1489..1530
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga tgaccaaggt actaggcatg
       61 gccccagttc tgggccctag gcctccacag gagcaggtgg ggcctctgat ggtaaaagtc
      121 gaggagaaag aagagaaagg caagtacctt cctagcctgg agatgttccg ccagcgcttc
      181 aggcagtttg ggtaccatga tacccctgga ccccgagagg ccctgagcca actccgggtg
      241 ctctgctgtg agtggctgag gcccgagatc cacaccaagg agcagatcct ggagctactg
      301 gtgctggagc agttcctgac catcctgccc caggagctcc aggcctgggt gcaggagcat
      361 tgcccggaga gcgctgaaga ggctgtcact ctcctcgaag atctggagcg ggaactggat
      421 gagccaggac accaggtctc aactcctcca aacgaacaga aaccggtgtg ggagaagata
      481 tcctcctcag gaactgcaaa ggaatccccg agcagcatgc agccacagcc cttggagacc
      541 agtcacaaat acgagtcttg ggggcccctg tacatccaag agtctggtga ggagcaggag
      601 ttcgctcaag atccaagaaa ggtccgagat tgcagattga gtacccagca cgaggaatca
      661 gcagatgagc agaaaggttc tgaagcagag gggctcaaag gggatataat ttctgtgatt
      721 atcgccaata aacctgaggc cagcttagag aggcagtgcg taaaccttga aaatgaaaaa
      781 ggaacaaaac cccctcttca agaggcaggc tccaagaaag gtagagaatc agttcctact
      841 aaacctaccc caggagagag acgttatata tgtgctgaat gtggcaaagc ctttagtaat
      901 agctcaaatc tcaccaaaca caggagaaca cacactgggg agaaacctta cgtgtgcacc
      961 aagtgtggga aagctttcag ccacagctca aacctcaccc tccactacag aacacacttg
     1021 gtggaccggc cctatgactg taagtgtgga aaagcttttg ggcagagctc agaccttctt
     1081 aaacatcaga gaatgcacac agaagaggcg ccatatcagt gcaaagattg tggcaaggct
     1141 ttcagcggga aaggcagcct cattcgtcac tatcggatcc acactgggga gaagccttat
     1201 cagtgtaacg aatgtgggaa gagcttcagt cagcatgcgg gcctcagctc ccaccagaga
     1261 ctccacaccg gagagaagcc atataagtgt aaggagtgtg ggaaagcctt caaccacagc
     1321 tccaacttca ataaacacca cagaatccac accggggaaa agccctactg gtgtcatcac
     1381 tgtggaaaga ccttctgtag caagtccaat ctttccaaac atcagcgagt ccacactgga
     1441 gagggagaag caccgtaccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1501 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1561 atttcgattt cttggcttta tatatcttgt ggaaaggacg acaacttggc ctgcctcggg
     1621 aaatt
//
