LOCUS       sequence-286680-        1493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3379
FEATURES             Location/Qualifiers
     source          1..1493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1325..1349)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1357..1398
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gcggcggcgt gtacggggga
       61 gatgaagttg gagcccttgt ttttgacatt ggatcctata ctgtgagagc tggttatgct
      121 ggtgaggact gccccaaggt ggattttcct acagctattg gtatggtggt agaaagagat
      181 gacggaagca cattaatgga aatagatggc gataaaggca aacaaggcgg tcccacctac
      241 tacatagata ctaatgctct gcgtgttccg agggagaata tggaggccat ttcacctcta
      301 aaaaatggga tggttgaaga ctgggatagt ttccaagcta ttttggatca tacctacaaa
      361 atgcatgtca aatcagaagc cagtctccat cctgttctca tgtcagaggc accgtggaat
      421 actagagcaa agagagagaa actgacagag ttaatgtttg aacactacaa catccctgcc
      481 ttcttccttt gcaaaactgc agttttgaca gcatttgcta atggtcgttc tactgggctg
      541 attttggaca gtggagccac tcataccact gcaattccag tccacgatgg ctatgtcctt
      601 caacaaggca ttgtgaaatc ccctcttgct ggagacttta ttactatgca gtgcagagaa
      661 ctcttccaag aaatgaatat tgaattggtt cctccatata tgattgcatc aaaagaagct
      721 gttcgtgaag gatctccagc aaactggaaa agaaaagaga agttgcctca ggttacgagg
      781 tcttggcaca attatatgtg taattgtgtt atccaggatt ttcaagcttc ggtacttcaa
      841 gtgtcagatt caacttatga tgaacaagtg gctgcacaga tgccaactgt tcattatgaa
      901 ttccccaatg gctacaattg tgattttggt gcagagcggc taaagattcc agaaggatta
      961 tttgaccctt ccaatgtaaa ggggttatca ggaaacacaa tgttaggagt cagtcatgtt
     1021 gtcaccacaa gtgttgggat gtgtgatatt gatatcagac caggtctcta tggcagtgta
     1081 atagtggcag gaggaaacac actaatacag agttttactg acaggttgaa tagagagctg
     1141 tctcagaaaa ctcctccaag tatgcggttg aaattgattg caaataatac aacagtggaa
     1201 cggaggttta gctcatggat tggcggctcc attctagcct ctttgggtac ctttcaacag
     1261 atgtggattt ccaagcaaga atatgaagaa ggagggaagc agtgtgtaga aagaaaatgc
     1321 ccttgcccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     1381 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1441 tggctttata tatcttgtgg aaaggacgac tgacagtaca gcgtcagtca tta
//
