LOCUS       sequence-286686-        1598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3381
FEATURES             Location/Qualifiers
     source          1..1598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1430..1454)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1462..1503
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg aaactgaaca gccagaagaa
       61 accttcccta acactgaaac caatggtgaa tttggtaaac gccctgcaga agatatggaa
      121 gaggaacaag catttaaaag atctagaaac actgatgaga tggttgaatt acgcattctg
      181 cttcagagca agaatgctgg ggcagtgatt ggaaaaggag gcaagaatat taaggctctc
      241 cgtacagact acaatgccag tgtttcagtc ccagacagca gtggccccga gcgcatattg
      301 agtatcagtg ctgatattga aacaattgga gaaattctga agaaaatcat ccctaccttg
      361 gaagagggcc tgcagttgcc atcacccact gcaaccagcc agctcccgct cgaatctgat
      421 gctgtggaat gcttaaatta ccaacactat aaaggaagtg actttgactg cgagttgagg
      481 ctgttgattc atcagagtct agcaggagga attattgggg tcaaaggtgc taaaatcaaa
      541 gaacttcgag agaacactca aaccaccatc aagcttttcc aggaatgctg tcctcattcc
      601 actgacagag ttgttcttat tggaggaaaa cccgataggg ttgtagagtg cataaagatc
      661 atccttgatc ttatatctga gtctcccatc aaaggacgtg cacagcctta tgatcccaat
      721 ttttacgatg aaacctatga ttatggtggt tttacaatga tgtttgatga ccgtcgcgga
      781 cgcccagtgg gatttcccat gcggggaaga ggtggttttg acagaatgcc tcctggtcgg
      841 ggtgggcgtc ccatgcctcc atctagaaga gattatgatg atatgagccc tcgtcgagga
      901 ccacctcccc ctcctcccgg acgaggcggc cggggtggta gcagagctcg gaatcttcct
      961 cttcctccac caccaccacc tagaggggga gacctcatgg cctatgacag aagagggaga
     1021 cctggagacc gttacgacgg catggttggt ttcagtgctg atgaaacttg ggactctgca
     1081 atagatacat ggagcccatc agaatggcag atggcttatg aaccacaggg tggctccgga
     1141 tatgattatt cctatgcagg gggtcgtggc tcatatggtg atcttggtgg acctattatt
     1201 actacacaag taactattcc caaagatttg gctggatcta ttattggcaa aggtggtcag
     1261 cggattaaac aaatccgtca tgagtcggga gcttcgatca aaattgatga gcctttagaa
     1321 ggatccgaag atcggatcat taccattaca ggaacacagg accagataca gaatgcacag
     1381 tatttgctgc agaacagtgt gaagcagtat gcagatgttg aaggattcta cccaactttc
     1441 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
     1501 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1561 tgtggaaagg acgaccccat tgtatataga ttgtgaat
//
