LOCUS       sequence-286689-        1580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3382
FEATURES             Location/Qualifiers
     source          1..1580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1412..1436)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1444..1485
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgtctcaggg gtcagtgaca
       61 ttcagagatg tggccataga cttctcccag gaggagtgga aatggcttca gcctgctcaa
      121 agagatttgt acagatgtgt aatgttggag aactatggcc atctggtctc actgggtctt
      181 tccatttcta agccagatgt ggtttcctta ttggagcaag ggaaagaacc ctggctgggg
      241 aaaagggaag tgaaaagaga tctgttttca gtttcagagt caagtggtga gatcaaagac
      301 ttttcaccaa aaaatgtcat ttatgatgac tcatcccagt atttgatcat ggaaagaatt
      361 ctaagtcaag gccctgtgta ttccagtttt aaaggaggct ggaaatgcaa ggatcatact
      421 gagatgctgc aagaaaatca gggatgtatt aggaaagtaa cagtctctca tcaagaagcc
      481 ctggctcaac atatgaatat cagtactgtg gagaggccct atggatgcca tgaatgtgga
      541 aaaacttttg gtcgacgctt ttccctggtg ttacaccaga ggactcatac tggagagaaa
      601 ccatatgcat gtaaggaatg tggcaaaacc tttagccaga tttcaaacct tgtgaaacac
      661 caaatgatac atactggaaa gaaaccccat gagtgtaagg actgtaataa aacattcagt
      721 tacctttcat ttcttattga acaccagaga acgcacactg gggagaaacc ttatgaatgt
      781 actgagtgtg gaaaggcctt tagccgtgcc tccaacctca ctcgacatca aagaattcac
      841 ataggaaaga aacaatatat atgtaggaaa tgtggtaaag catttagcag tggctcagaa
      901 ctcattcgcc accagattac acatactgga gagaaacctt atgaatgcat tgaatgtggg
      961 aaggcatttc gccgtttctc acaccttact cgacatcaga gcatccatac aaccaaaacc
     1021 ccgtatgaat gtaatgaatg taggaaagct ttccgttgtc actcattcct tattaaacat
     1081 cagagaattc atgctggaga aaagctctat gaatgtgatg aatgtggtaa agttttcact
     1141 tggcatgcat cccttattca acatacgaag agtcacactg gagagaaacc ctatgcgtgt
     1201 gctgaatgtg ataaagcctt cagccggagc ttttccctca ttctacatca gagaactcat
     1261 actggagaga aaccctatgt atgtaaggta tgcaacaaat ccttcagctg gagctcaaac
     1321 cttgctaaac atcagaggac acacactctt gacaacccct atgaatatga aaattcattt
     1381 aattaccact cattccttac tgaacaccag ttgccaactt tcttgtacaa agtggttgat
     1441 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     1501 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgagtta
     1561 taggtccagc taccggctca
//
