LOCUS       sequence-286692-        1148 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3383
FEATURES             Location/Qualifiers
     source          1..1148
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(980..1004)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1012..1053
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggaggctgg accctggaga
       61 gtttctgcac ccccttcagg acccccacag ttccccgctg tggtccctgg accatcgctg
      121 gaggtggccc gagctcacat gctggctttg gggccacagc agctgctggc ccaggatgag
      181 gagggggaca cgctccttca cctgtttgcg gctcgggggc tgcgctgggc ggcatatgct
      241 gcggctgagg tgctccaggt gtaccggcgt cttgacattc gtgagcataa gggcaagacc
      301 cctctcctgg tggcggctgc tgccaaccag cccctgattg tggaggatct gttgaacctg
      361 ggagcagagc ccaatgccgc tgaccatcag ggacgttcgg tcttgcacgt ggccgctacc
      421 tacgggctcc caggagttct cttggctgtg cttaactctg gggtccaggt tgacctggaa
      481 gccagagact tcgagggcct caccccgctc cacacggcca tcctggccct taacgttgct
      541 atgcgccctt ccgacctctg tccccgggtg ctgagcacac aggcccgaga caggctggat
      601 tgtgtccaca tgttgctgca aatgggtgct aatcacacca gccaggagat caagagcaac
      661 aagacagttc tgcacttggc cgtgcaggct gccaacccca ctctggttca gctgctgctg
      721 gagctgcccc ggggagacct gcggaccttt gtcaacatga aggcccacgg gaacacagcc
      781 ctccacatgg cggctgccct gccccctggg ccggcccagg aggccatcgt gcggcacctg
      841 ttggcagctg gggcggaccc cacactgcgc aacctggaga atgagcagcc cgttcacctg
      901 ctgcggcccg ggccgggccc tgaggggctc cggcagctgt tgaagaggag ccgtgtggcg
      961 ccgccaggcc tgtcctcttt gccaactttc ttgtacaaag tggttgatat cggtaagcct
     1021 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
     1081 agtatttcga tttcttggct ttatatatct tgtggaaagg acgatcgatc tttatcttca
     1141 tcaagcaa
//
