LOCUS       sequence-286701-        1559 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3386
FEATURES             Location/Qualifiers
     source          1..1559
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1391..1415)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1423..1464
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga acctggaggg cggcggccga
       61 ggcggagagt tcggcatgag cgcggtgagc tgcggcaacg ggaagctccg ccagtggctg
      121 atcgaccaga tcgacagcgg caagtacccc gggctggtgt gggagaacga ggagaagagc
      181 atcttccgca tcccctggaa gcacgcgggc aagcaggact acaaccgcga ggaggacgcc
      241 gcgctcttca aggcttgggc actgtttaaa ggaaagttcc gagaaggcat cgacaagccg
      301 gaccctccca cctggaagac gcgcctgcgg tgcgctttga acaagagcaa tgactttgag
      361 gaactggttg agcggagcca gctggacatc tcagacccgt acaaagtgta caggattgtt
      421 cctgagggag ccaaaaaagg agccaagcag ctcaccttgg aggacccgca gatgtccatg
      481 agccacccct acaccatgac aacgccttac ccttcgctcc cagcccagca ggttcacaac
      541 tacatgatgc cacccctcga ccgaagctgg agggactacg tcccggatca gccacacccg
      601 gaaatcccgt accaatgtcc catgacgttt ggaccccgcg gccaccactg gcaaggccca
      661 gcttgtgaaa atggttgcca ggtgacagga accttttatg cttgtgcccc acctgagtcc
      721 caggctcccg gagtccccac agagccaagc ataaggtctg ccgaagcctt ggcgttctca
      781 gactgccggc tgcacatctg cctgtactac cgggaaatcc tcgtgaagga gctgaccacg
      841 tccagccccg agggctgccg gatctcccat ggacatacgt atgacgccag caacctggac
      901 caggtcctgt tcccctaccc agaggacaat ggccagagga aaaacattga gaagctgctg
      961 agccacctgg agaggggcgt ggtcctctgg atggcccccg acgggctcta tgcgaaaaga
     1021 ctgtgccaga gcaggatcta ctgggacggg cccctggcgc tgtgcaacga ccggcccaac
     1081 aaactggaga gagaccagac ctgcaagctc tttgacacac agcagttctt gtcagagctg
     1141 caagcgtttg ctcaccacgg ccgctccctg ccaagattcc aggtgactct atgctttgga
     1201 gaggagtttc cagaccctca gaggcaaaga aagctcatca cagctcacgt agaacctctg
     1261 ctagccagac aactatatta ttttgctcaa caaaacagtg gacatttcct gaggggctac
     1321 gatttaccag aacacatcag caatccagaa gattaccaca gatctatccg ccattcctct
     1381 attcaagaat gcccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     1441 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1501 atttcttggc tttatatatc ttgtggaaag gacgacttgc gcacagcttt tttacgtga
//
