LOCUS       sequence-286704-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3387
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(671..695)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             703..744
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcggcagc agccgctctg
       61 agggcgcctg ctcaggttgt tggcatggag gggaagatga ggcagcacct tctaagcaga
      121 gcacgtgtat acatatatac aaagaccagg gaggtcatag tggagaaagg ccttatgagt
      181 gtggggaata taggaaatta tttaagaaca agtcctgcct cactgaaccc agaagagatc
      241 acaaacacag gaatgttcgc actggagaaa ggccttatga gtgcagcaaa tatgggaaat
      301 tatttcacca aaagcctaca ctccatattc atgagagatt tcatactggg caaaagacct
      361 atgagtgcag tgagtgtgga aaatcatttc accaaagctc ttcactcttg cagcgtcaga
      421 cacttcacac tagagaaagg ccttatgagt gtattgaatg tgggaaagcc tttgctgaaa
      481 agtccagtct cattaaccac aggaaagttc actctggagc aaagcgttat gaatgcaatg
      541 aatgtgggaa gtcctttgct tatacatcta gtctcattaa acacaggagg attcacactg
      601 gagagaggcc ttatgagtgc agtgaatgtg ggagatcctt tgctgaaaac tccagtctta
      661 ttaaacactt tgccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
      721 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
      781 atttcttggc tttatatatc ttgtggaaag gacgagctgt tgtgtgtcat ccagcatca
//
