LOCUS       sequence-286707-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3388
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(720..744)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             752..793
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttgggcatg ttcaagagaa tggccgaatt
       61 tgggcctgac tccggcggga gagtaaaggg tgttactatc gttaaaccaa tagtttacgg
      121 taatgttgct cggtattttg gaaagaaaag agaagaagat gggcacactc atcagtggac
      181 agtatatgtg aaaccatata gaaatgagga tatgtcagca tatgtgaaga aaatccagtt
      241 taaattacat gaaagctatg gcaatccttt aagagttgtt actaaacctc catatgaaat
      301 tactgaaaca ggatggggtg aattcgaaat aatcatcaaa atatttttca ttgaccctaa
      361 tgaaagacct gtaaccctgt atcatttgct aaagctgttt caatcagaca ccaatgcaat
      421 gctggggaaa aagacagtgg tttcagagtt ctatgatgaa atgatatttc aagacccaac
      481 agcaatgatg caacaattat tgacaacatc tcgtcagcta acattaggag cctataagca
      541 tgaaacagaa tttgcagagc ttgaagtgaa aaccagagaa aaattagaag ctgctaagaa
      601 aaaaacaagc tttgagattg cagagcttaa ggagagatta aaagcaagtc gtgaaactat
      661 aaattgttta aaaaatgaaa tcagaaaact tgaagaagat gaccaagcaa aagacatata
      721 cccaactttc ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg
      781 tctcgattct acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct
      841 ttatatatct tgtggaaagg acgagtattt aaagattcga accaatta
//
