LOCUS       sequence-286710-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3389
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(554..578)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             586..627
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc tgctgctgcc gccgcggcca
       61 ccccaccctc ggtcctcctc tccggaggcc atggacccac cgccccccaa ggctccccct
      121 ttccccaagg cggaaggccc ctcctccact ccttcctcgg cggcggggcc ccgacccccg
      181 cggctgggcc gccacctcct catcgacgcc aatggggtcc cctacacata cacggtgcag
      241 ctggaggagg agccccgggg cccgccccag cgcgaggcgc ccccaggaga gcccggccct
      301 cgcaagggct acagctgccc ggagtgcgcc cgtgtctttg ccagccctct gcggctgcag
      361 agccaccgcg tgtcgcactc ggacctcaag cccttcacgt gcggcgcctg cggcaaggcc
      421 ttcaagcgct ccagccacct gtcgcggcat cgcgccacgc accgcgcccg cgccgggccg
      481 ccgcacacct gcccgctctg tccacgccgc ttccaggacg ccgcggagct ggcgcagcac
      541 gtgcgcctcc actacccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
      601 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
      661 tcgatttctt ggctttatat atcttgtgga aaggacgatc cattgcccga gttggggctg
      721 tc
//
