LOCUS       sequence-286713-        1601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3390
FEATURES             Location/Qualifiers
     source          1..1601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1433..1457)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1465..1506
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga cgtcaatggc cagcttgttt
       61 tcttttacta gtccagcagt aaagcgattg ttgggctgga aacaaggtga tgaggaggag
      121 aaatgggcag aaaaggcagt tgatgctttg gtgaagaaac taaaaaagaa aaagggtgcc
      181 atggaggaac tggagaaagc cttgagcagt ccaggacagc cgagtaaatg tgtcactatt
      241 cccagatctt tagatggacg cctgcaggtt tctcacagaa aaggcttacc ccatgttata
      301 tattgtcgtg tttggcgctg gccggatttg cagagtcatc atgagctaaa gccgttggat
      361 atttgtgaat ttccttttgg atctaagcaa aaagaagttt gtatcaaccc ataccactat
      421 aagagagtgg agagtccagt cttacctcca gtattagtgc ctcgtcataa tgaattcaat
      481 ccacaacaca gccttctggt tcagtttagg aacctgagcc acaatgaacc acacatgcca
      541 caaaatgcca cgtttccaga ttctttccac cagcccaaca acactccttt tcccttatct
      601 ccaaacagcc cttatccccc ttctcctgct agcagcacat atcccaactc cccagcaagt
      661 tctggaccag gaagtccatt tcagctccca gctgatacgc ctcctcctgc ctatatgcca
      721 cctgatgatc agatgggtca agataattcc cagcctatgg atacaagcaa taatatgatt
      781 cctcagatta tgcccagtat atccagcagg gatgttcagc ctgttgccta tgaagagcct
      841 aaacattggt gttcaatagt ctactatgaa ttaaacaatc gtgttggaga agcttttcat
      901 gcatcttcta ctagtgtgtt agtagatgga ttcacagatc cttcaaataa caaaagtaga
      961 ttctgcttgg gtttgttgtc aaatgttaat cgtaattcga caattgaaaa cactaggcga
     1021 catattggaa aaggtgttca tctgtactat gttggtggag aggtgtatgc ggaatgcctc
     1081 agtgacagca gcatatttgt acagagtagg aactgcaact ttcatcatgg ctttcatccc
     1141 accactgtct gtaagattcc cagcagctgc agcctcaaaa tttttaacaa tcaggagttt
     1201 gctcagcttc tggctcaatc tgtcaaccat gggtttgagg cagtatatga gctcaccaaa
     1261 atgtgtacca ttcggatgag ttttgtcaag ggttggggag cagaatatca ccggcaggat
     1321 gtaaccagca ccccatgttg gattgagatt catcttcatg ggcctcttca gtggctggat
     1381 aaagtcctta ctcagatggg ctcccctctg aaccccatat cttctgtttc atacccaact
     1441 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     1501 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1561 tcttgtggaa aggacgaccc ataccggcaa atactaccac t
//
