LOCUS       sequence-286716-         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3391
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(548..572)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             580..621
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa tttggcatga gcagcaatga gtgcttcaag
       61 tgtggacgat ctggccactg ggcccgggaa tgtcctactg gtggaggccg tggtcgtgga
      121 atgagaagcc gtggcagagg tttccagttt gtttcctcgt ctcttccaga catttgttat
      181 cgctgtggtg agtctggtca tcttgccaag gattgtgatc ttcaggagga tgcctgctat
      241 aactgcggta gaggtggcca cattgccaag gactgcaagg agcccaagag agagcgagag
      301 caatgctgct acaactgtgg caaaccaggc catctggctc gtgactgcga ccatgcagat
      361 gagcagaaat gctattcttg tggagaattc ggacacattc aaaaagactg caccaaagtg
      421 aagtgctata ggtgtggtga aactggtcat gtagccatca actgcagcaa gacaagtgaa
      481 gtcaactgtt accgctgtgg cgagtcaggg caccttgcac gggaatgcac aattgaggct
      541 acagcctacc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
      601 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
      661 tcttggcttt atatatcttg tggaaaggac gatcacacca ccctttgccc ggcggt
//
