LOCUS       sequence-286722-        1205 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3393
FEATURES             Location/Qualifiers
     source          1..1205
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1037..1061)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1069..1110
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cagaaacatc gccagagcct
       61 tctgggcagc ttgttgtaca ctcagacgct cacagtgaca ctgtgctggc cagttttgag
      121 gatcagagga agaaaggctt cctctgtgac attactttaa tcgtggagaa tgtacatttc
      181 cgggcccaca aagccttact tgctgccagt agtgaatact tctcaatgat gtttgcagaa
      241 gagggggaaa tcggccaatc catttatatg ctggaaggca tggttgcaga cacctttggt
      301 atcctgctgg aatttatcta cacaggttat ctccatgcca gtgagaaaag tacagaacaa
      361 atcctggcta ctgctcagtt cttaaaagtc tatgacctgg taaaggctta cacagacttc
      421 caaaataatc atagctcccc aaagccaaca actttgaaca ctgctggtgc cccagtggtt
      481 gttatctcta ataagaaaaa cgatcctcca aagcggaaac ggggaagacc aaaaaaagtc
      541 aatacattgc aggaggagaa atcagaactg gctgcagagg aagaaataca gttaagagtg
      601 aacaattcag ttcagaatag acaaaacttt gtggttaaag gagacagtgg tgtactgaat
      661 gagcaaattg cagcaaaaga aaaggaagaa tcggagccta cttgtgagcc aagtagagag
      721 gaggaaatgc cagttgaaaa agatgagaac tatgatccca agaccgagga tggccaggca
      781 agccagagtc gatacagcaa gcggaggatt tggagatccg tcaaacttaa agattacaaa
      841 cttgttgggg atcaagagga ccatggttca gccaagagga tctgtggaag gagaaagcgc
      901 cctggaggcc ctgaggcccg ctgtaaagac tgtggcaagg tctttaagta caatcacttt
      961 ttagcaatcc accagaggag ccacacaggt aatgatgttt tcaaagctga ttgcagtgtg
     1021 ttgcagaatt gggagtaccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1081 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1141 atttcgattt cttggcttta tatatcttgt ggaaaggacg acataccccg ggcccccagc
     1201 acacc
//
