LOCUS       sequence-286725-        2300 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3394
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2132..2156)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2164..2205
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcagaaac atcgccagag
       61 ccttctgggc agcttgttgt acactcagac gctcacagtg acactgtgct ggccagtttt
      121 gaggatcaga ggaagaaagg cttcctctgt gacattactt taatcgtgga gaatgtacat
      181 ttccgggccc acaaagcctt acttgctgcc agtagtgaat acttctcaat gatgtttgca
      241 gaagaggggg aaatcggcca atccatttat atgctggaag gcatggttgc agacaccttt
      301 ggtatcctgc tggaatttat ctacacaggt tatctccatg ccagtgagaa aagtacagaa
      361 caaatcctgg ctactgctca gttcttaaaa gtctatgacc tggtaaaggc ttacacagac
      421 ttccaaaata atcatagctc cccaaagcca acaactttga acactgctgg tgccccagtg
      481 gttgttatct ctaataagaa aaacgatcct ccaaagcgga aacggggaag accaaaaaaa
      541 gtcaatacat tgcaggagga gaaatcagaa ctggctgcag aggaagaaat acagttaaga
      601 gtgaacaatt cagttcagaa tagacaaaac tttgtggtta aaggagacag tggtgtactg
      661 aatgagcaaa ttgcagcaaa agaaaaggaa gaatcggagc ctacttgtga gccaagtaga
      721 gaggaggaaa tgccagttga aaaagatgag aactatgatc ccaagaccga ggatggccag
      781 gcaagccaga gtcgatacag caagcggagg atttggagat ccgtcaaact taaagattac
      841 aaacttgttg gggatcaaga ggaccatggt tcagccaaga ggatctgtgg aaggagaaag
      901 cgccctggag gccctgaggc ccgctgtaaa gactgtggca aggtctttaa gtacaatcac
      961 tttttagcaa tccaccagag gagccacaca ggggagcgac ctttcaaatg taatgagtgt
     1021 ggaaaaggct ttgcccagaa gcactcgcta caggtccaca ccaggatgca cacaggcgag
     1081 cggccgtaca cctgcaccgt gtgcagcaag gctctgacca ccaagcactc actgctggag
     1141 cacatgagcc tgcactcagg acagaagtct tttacctgtg atcaatgcgg aaaatatttc
     1201 agccagaaca gacagctaaa gagccattac cgagttcata caggccactc attaccggaa
     1261 tgcaaagact gccatcgcaa attcatggat gtgtctcagc taaagaaaca tctgcgaaca
     1321 cacacaggtg agaagccatt tacttgtgaa atctgtggca aatctttcac agcaaagagt
     1381 tctcttcaga cccacatcag aatccatcga ggagaaaagc catactcctg tggcatttgt
     1441 ggcaaatcct tctctgactc cagtgccaaa aggagacact gcattctaca cactggcaag
     1501 aagcctttct cctgccctga gtgtaactta cagtttgctc gcttagacaa cttgaaggct
     1561 cacttgaaaa ttcatagcaa ggagaagcat gcttcagatg ccagcagcat ttctggcagt
     1621 agtaatacag aagaggtcag gaatattctt cagctacagc catatcaact ctctacctcg
     1681 ggagagcagg aaattcagct tctcgtaacc gattctgtac ataacatcaa tttcatgccc
     1741 ggtcctagcc agggaatcag cattgtgact gcagagagtt cccaaaacat gactgcagac
     1801 caggctgcta atcttaccct gctcacgcag cagccagagc aactgcagaa tttaattctt
     1861 tcagctcaac aggagcaaac agaacacatt cagagcctca atatgattga aagccagatg
     1921 gggccctcac aaacagagcc agtgcacgtg atcactctgt ccaaggaaac actggaacat
     1981 cttcatgccc atcaagagca aacagaggag ctccatttag ctacaagtac ttcagatcca
     2041 gctcagcacc tgcagctgac acaggagcct ggtccgccac cacccactca ccacgtgccc
     2101 cagccaacgc cacttggcca ggagcagagc ttgccaactt tcttgtacaa agtggttgat
     2161 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     2221 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgagatg
     2281 catttggttt gtctgccctc
//
