LOCUS       sequence-286728-        1766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3395
FEATURES             Location/Qualifiers
     source          1..1766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1598..1622)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1630..1671
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctctgagctc tcgggcgcgt
       61 gccttctccg tggaagcctt ggtggggaga cccagcaaaa gaaaactcca agacccaata
      121 caggcggagc agcctgagct gcgggagaaa aagggcggag aggaagagga ggagagaagg
      181 agcagcgctg cagggaagag cgagccgctt gaaaaacaac ctaagacaga gccctcaaca
      241 tctgcttcct ctggctgcgg cagcgacagc ggctacggca acagctctga aagtctggaa
      301 gagaaagata ttcaaatgga gcttcaagga tctgaactgt ggaaaagatt ccatgacatc
      361 gggactgaga tgatcattac taaagcgggc aggcggatgt tcccctctgt tcgggtcaag
      421 gtgaaagggt tggatccagg gaagcagtac catgtggcca tcgatgtggt gccggtggat
      481 tccaaacgct ataggtacgt ctatcacagc tcacagtgga tggtagctgg gaatacagac
      541 catttgtgca tcattcctag attctatgtt cacccggact caccctgctc gggagagacc
      601 tggatgcggc agatcatcag ctttgatcgc atgaaactca ccaacaatga gatggatgac
      661 aaaggccaca tcattctgca atccatgcat aagtacaaac cccgagtgca cgtgatagag
      721 caaggcagca gtgttgacct gtcccagatt cagtccttgc ccactgaagg tgttaaaaca
      781 ttctccttta aagaaactga gttcaccaca gtaacggctt accaaaacca acagattacg
      841 aaactaaaaa tagaaagaaa tccttttgct aaaggattta gagatactgg aagaaacagg
      901 ggtgtattgg atgggctttt agagacctac ccatggaggc cttctttcac tctcgatttt
      961 aaaacctttg gcgcagacac acaaagtgga agcagtggct catctccagt gacctctagt
     1021 ggaggggccc cctctccttt gaactcctta ctttctccac tttgcttttc acctatgttt
     1081 catttaccta caagctccct tggaatgccc tgtccagagg catacctgcc caatgtcaac
     1141 ctgcctctat gctacaagat ttgtccaact aatttttggc aacagcaacc tcttgtttta
     1201 ccggctcctg aaagactagc aagcagcaac agttctcagt ctttagcccc actcatgatg
     1261 gaagtgccta tgttatcttc cctgggggtc accaattcaa aaagcggttc atctgaagac
     1321 tccagtgatc agtatctaca agcacctaat tctaccaatc aaatgttata tggattacag
     1381 tcacctggaa atatttttct gccaaactcc atcaccccag aagcacttag ttgctccttt
     1441 catccttcct atgactttta tagatacaat ttctctatgc catctagact gataagtggt
     1501 tccaaccatc ttaaagtgaa tgacgacagt caagtttctt ttggagaagg caaatgtaat
     1561 catgttcatt ggtatccagc aattaaccat tacctttacc caactttctt gtacaaagtg
     1621 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     1681 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1741 gagagtcgct ccagcttctt acaaca
//
