LOCUS       sequence-28673-e         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Unsal-Kacmaz K, Chastain PD, Qu PP, Minoo P, Cordeiro-Stone M, 
            Sancar A, Kaufmann WK
  TITLE     The human Tim/Tipin complex coordinates an Intra-S checkpoint 
            response to UV that slows replication fork displacement.
  JOURNAL   Mol Cell Biol. 2007 Apr . 27(8):3131-42.
  PUBMED    17296725
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Apr . 27(8):3131-42."
COMMENT     Sequence Label: pFastBac-His-Flag-Timeless
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             92..109
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             131..151
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             227..250
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca ccatcgggcg
       61 cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacg attacgatat
      121 cccaacgacc gaaaacctgt attttcaggg cgccatggga tccggaattc aaaggcctac
      181 gtcgacgagc tcactagtcg cggccgcttt cgaatctaga aggatggact acaaggacga
      241 cgatgacaag gacttgcaca tgatgaactg tgaacttcta gccacatgta gtgcccttgg
      301 gtacttggag ggagacactt accataagga accagattgc ttagagagcg tgaaggatct
      361 gatccgctat ttgaggcatg aggatgagac acgagatgtg cggcagcagc tgggggcagc
      421 ccagatccta cagagcgacc ttctgcccat cctcacccag caccaccagg acaagcctct
      481 ctttgatgct gttatcagac tgatggtgaa cttgacacaa ccagccttgc tctgttttgg
      541 caatctgcct aaggagccca gctttcggca ccattttttg caggtgctaa cttatttgca
      601 ggcctacaaa gaggc
//
