LOCUS       sequence-286734-        1361 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3397
FEATURES             Location/Qualifiers
     source          1..1361
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1193..1217)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1225..1266
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgtgtgtccc agtcctgaaa
       61 gatattttgc atctagctga tctccctggg cagaaaccat acttggttgg agaatgtaca
      121 aaccatcacc agcaccagaa gcatcacagt gcaaagaaat ccttgaagag ggacatggac
      181 agagcctcat atgtgaagtg ctgcctattc tgtatgtcat tgaagccctt tcgcaaatgg
      241 gaggttggaa aggaccttcc agccatgttg cggcttctga ggtccctggt ctttcctgga
      301 ggcaagaaac ccggcacaat tactgaatgt ggggaggaca ttcgcagtca aaaaagtcat
      361 tacaagtcag gtgaatgtgg gaaggcttcc aggcacaaac acactcctgt ttaccatcca
      421 agagtctaca ctggaaaaaa gctttatgag tgtagcaaat gtgggaaagc cttccgtggc
      481 aagtactcac ttgttcagca ccagagagtc catactggag aaaggccttg ggagtgcaat
      541 gaatgtggaa aattctttag ccaaacctcc cacctgaatg atcatcggag aatccacacc
      601 ggagaaaggc cttatgagtg cagcgaatgt ggaaaattat ttagacaaaa ctccagcctt
      661 gttgaccacc agaaaataca cactggagca aggccttatg agtgtagcca gtgtgggaaa
      721 tcctttagcc aaaaagccac ccttgttaaa caccaaagag ttcacactgg agaaaggcct
      781 tataagtgtg gtgaatgtgg gaattccttt agtcaaagtg ccattcttaa tcaacaccga
      841 agaattcaca ctggagcaaa gccttatgag tgtggccagt gtgggaaatc ctttagtcaa
      901 aaagctaccc tcattaaaca ccagagagtt cacactggag aaaggcctta taagtgtggt
      961 gactgtggga aatcctttag tcaaagctcc atccttattc aacaccggag aattcatact
     1021 ggagcaaggc cttatgagtg tggccagtgt ggaaagtcct ttagccaaaa gtctggtctc
     1081 attcaacacc aagtggttca cactggagaa aggccttatg agtgcaacaa atgtgggaat
     1141 tcctttagcc aatgctccag cctcatacat caccaaaaat gtcataacac attgccaact
     1201 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     1261 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1321 tcttgtggaa aggacgaagt tattagtaat aatttaaacc t
//
