LOCUS       sequence-286737-        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3398
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(923..947)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             955..996
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg agaaaatgtc ccgaccgctc
       61 cccctgaatc ccacctttat cccgcctccc tacggcgtgc tcaggtccct gctggagaac
      121 ccgctgaagc tcccccttca ccacgaagac gcatttagta aagataaaga caaggaaaag
      181 aagctggatg atgagagtaa cagcccgacg gtcccccagt cggcattcct ggggcctacc
      241 ttatgggaca aaacccttcc ctatgacgga gatactttcc agttggaata catggacctg
      301 gaggagtttt tgtcagaaaa tggcattccc cccagcccat ctcagcatga ccacagccct
      361 caccctcctg ggctgcagcc agcttcctcg gctgccccct cggtcatgga cctcagcagc
      421 cgggcctctg caccccttca ccctggcatc ccatctccga actgtatgca gagccccatc
      481 agaccaggtc agctgttgcc agcaaaccgc aatacaccaa gtcccattga tcctgacacc
      541 atccaggtcc cagtgggtta tgagccagac ccagcagatc ttgccctttc cagcatccct
      601 ggccaggaaa tgtttgaccc tcgcaaacgc aagttctctg aggaagaact gaagccacag
      661 cccatgatca agaaagctcg caaagtcttc atccctgatg acctgaagga tgacaagtac
      721 tgggcaaggc gcagaaagaa caacatggca gccaagcgct cccgcgacgc ccggaggctg
      781 aaagagaacc agatcgccat ccgggcctcg ttcctggaga aggagaactc ggccctccgc
      841 caggaggtgg ctgacttgag gaaggagctg ggcaaatgca agaacatact tgccaagtat
      901 gaggccaggc acgggcccct gtacccaact ttcttgtaca aagtggttga tatcggtaag
      961 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1021 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgatcc caaggagtgg
     1081 aagacatatg g
//
