LOCUS       sequence-286740-        1406 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3399
FEATURES             Location/Qualifiers
     source          1..1406
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1238..1262)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1270..1311
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ttcactccag catgggggct
       61 ccagaaataa gaatgtctaa gcccctggag gccgagaagc aaggtctgga ctccccatca
      121 gagcacacag acaccgaaag aaatggacca gacactaatc atcagaaccc ccaaaataag
      181 acctccccat tctccgtgtc cccaactggc cccagtacaa agatcaaggc tgaagacccc
      241 agtggcgatt cagccccagc agcacccctg ccccctcagc cggcccagcc tcatctgccc
      301 caggcccaac tcatgttgac gggcagccag ctagctgggg acatacagca gctcctccag
      361 ctccagcagc tggtgcttgt gccaggccac cacctccagc cacctgctca gttcctgcta
      421 ccgcaggccc agcagagcca gccaggcctg ctaccgacac caaatctatt ccagctacct
      481 cagcaaaccc agggagctct tctgacctcc cagccccggg ccgggcttcc cacacagccc
      541 cccaaatgct tggagccacc atcccacccc gaggagccca gtgatctgga ggagctggag
      601 caattcgccc gcaccttcaa gcaacgccgc atcaagctgg gcttcacgca gggtgatgtg
      661 ggcctggcca tgggcaagct ctacggcaac gacttcagcc agacgaccat ttcccgcttc
      721 gaggccctca acctgagctt caagaacatg tgcaaactca agcccctcct ggagaagtgg
      781 ctcaacgatg cagagactat gtctgtggac tcaagcctgc ccagccccaa ccagctgagc
      841 agccccagcc tgggtttcga cggcctgccc ggccggagac gcaagaagag gaccagcatc
      901 gagacaaacg tccgcttcgc cttagagaag agttttctag cgaaccagaa gcctacctca
      961 gaggagatcc tgctgatcgc cgagcagctg cacatggaga aggaagtgat ccgcgtctgg
     1021 ttctgcaacc ggcgccagaa ggagaaacgc atcaacccct gcagtgcggc ccccatgctg
     1081 cccagcccag ggaagccggc cagctacagc ccccatatgg tcacacccca agggggcgcg
     1141 gggaccttac cgttgtccca agcttccagc agtctgagca caacagcaca aaccccagcc
     1201 ctcaaggcag ccactcggct atcggcttgt caggcctgcc caactttctt gtacaaagtg
     1261 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     1321 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1381 gaatcggtgc cccacatgac ggccca
//
