LOCUS       sequence-286743-        1895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3400
FEATURES             Location/Qualifiers
     source          1..1895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1727..1751)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1759..1800
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctaatgcaga agtgagtgtc
       61 ccagtggggg atgtggttgt ggtacctact gaaggaaatg aaggggagaa tcctgaagac
      121 actaaaaccc aagtgatttt gcagttacag cctgtgcaac aagggattta tgaagctggg
      181 tcggagaaca acacggcagt tgtagcagta gaaactcaca cgatacacaa aattgaagaa
      241 gggattgata caggcactat agaagcaaat gaggatatgg aaattgctta ccccataact
      301 tgtggggaga gcaaagccat cctcctctgg aagaagtttg tatgtccagg aataaacgtg
      361 aagtgtgtca agttcaatga tcagttgatc agccccaagc actttgttca tctggctggc
      421 aagtccactc tgaaggactg gaagagagct attcgtctgg gtgggatcat gctcaggaaa
      481 atgatggact ccggacagat tgatttttac caacatgaca aagtttgctc caatacctgc
      541 agaagcacca aatttgatct tctgatcagc agtgcaagag ctccagtgcc aggacagcag
      601 acaagtgtgg tgcagacacc cacttcggct gatggtagca tcacgcagat tgccatctca
      661 gaagagagca tggaagaggc agggctggaa tggaactcag ctctcaccgc tgctgtcacc
      721 atggccacgg aggagggtgt aaagaaagac tcagaggaaa tttcagagga cactttgatg
      781 ttctggaaag gaatagctga tgtagggctg atggaagagg ttgtctgcaa tatacagaag
      841 gaaatagagg agctactcag gggagttcag cagcggctca tccaggctcc cttccaagtc
      901 acagatgctg ctgttctcaa caatgtagca cacacatttg gcctaatgga cacagtcaag
      961 aaggttttag acaacagaag gaaccaagta gagcagggag aagaacagtt tctctatact
     1021 ctgacagact tggaacgcca gttggaggag cagaagaagc aaggccagga tcacaggctg
     1081 aaatctcaga cagttcaaaa tgtggtactg atgcctgtga gcactcctaa gcctccaaaa
     1141 aggccccggc tccagcggcc agcctccacc actgtcttga gcccttctcc tcctgtccag
     1201 cagcctcagt tcacagtcat ctcacccatc accatcaccc cagtgggtca gtcattttcc
     1261 atgggcaata ttccagtggc caccctcagc cagggctcca gtcctgtgac tgtccacaca
     1321 ctgccttctg gccctcagct cttccgctat gccacagtgg tctcctctgc caagagcagc
     1381 tcaccagaca cagtgaccat ccacccttca tctagcttgg cgctgctgag ctctactgcc
     1441 atgcaggatg ggagtacact gggcaacatg accaccatgg ttagccctgt ggaattggtg
     1501 gccatggagt ccggcctaac ctcggcaatt caggctgttg aaagcacctc agaggatggg
     1561 cagaccatca ttgagattga tccagccccg gacccagaag ctgaagatac tgagggcaaa
     1621 gcagtcatct tggagacaga gctgaggact gaggagaaag ttgtggctga gatggaagaa
     1681 caccagcatc aagttcacaa tgtggagatt gtggtcttag aggattaccc aactttcttg
     1741 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     1801 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1861 ggaaaggacg aaaattggta ggtctcgctt gaata
//
