LOCUS       sequence-286749-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3402
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(680..704)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             712..753
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cacatgtttc ttcagaaact
       61 caagatgttt cccccaaaga tgaattaact gcttcagaag cctccactag gtctccattg
      121 tgtgaacaca ccttccctgg ggactcagac ttacggtcaa tgattgaaga acatgctttt
      181 caggttttgt cacaaggatc cttgttagaa agtccaagtt acacagtttg tgtctctgag
      241 ccagataaag atgatgattt tctttctctg aactttccca ggaaactttg gaaaatagtg
      301 gaaagtgacc aattcaagtc tatttcatgg gatgagaatg gaacttgcat agtgattaat
      361 gaagaactct tcaagaaaga aattttggaa acaaaggctc cttacagaat atttcaaact
      421 gatgctatca aaagttttgt tcgacagctc aacctttatg gatttagtaa aattcaacag
      481 aattttcaaa gatctgcctt tctagccacc tttctgtcag aagagaaaga atcgtctgtc
      541 ttaagcaagc ttcatgtctt tgtgtttcac cacagtctat ttggagcaac attcggagaa
      601 catcactttc agccatgccc tttaccatct ggtaacaagg ctcccatttc tacttggagt
      661 ggagattcct ggttgctgtg cccaactttc ttgtacaaag tggttgatat cggtaagcct
      721 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
      781 agtatttcga tttcttggct ttatatatct tgtggaaagg acgatcacac gtattcgagt
      841 gcgcggtc
//
