LOCUS       sequence-28675-e         624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Unsal-Kacmaz K, Chastain PD, Qu PP, Minoo P, Cordeiro-Stone M, 
            Sancar A, Kaufmann WK
  TITLE     The human Tim/Tipin complex coordinates an Intra-S checkpoint 
            response to UV that slows replication fork displacement.
  JOURNAL   Mol Cell Biol. 2007 Apr . 27(8):3131-42.
  PUBMED    17296725
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Apr . 27(8):3131-42."
COMMENT     Sequence Label: pFastBac-His-Flag-Tipin
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             93..110
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             132..152
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             171..194
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc
       61 gcggatctcg gtccgaaacc atgtcgtact accatcacca tcaccatcac gattacgata
      121 tcccaacgac cgaaaacctg tattttcagg gcgccatggg atccaggatg gactacaagg
      181 acgacgatga caagctagaa ccacaggaga atggcgtgat tgacctacca gattatgagc
      241 atgtagaaga tgaaactttt cctcctttcc cacctccagc ctctccagag agacaagatg
      301 gtgaaggaac tgagcctgat gaagagtcag gaaatggagc acctgttcct gtacctccaa
      361 agagaacagt taaaagaaat atacccaagc tggatgctca gagattaatt tcagagagag
      421 gacttccagc cttaaggcat gtatttgata aggcaaaatt caaaggtaaa ggtcatgagg
      481 ctgaagactt gaagatgcta atcagacaca tggagcactg ggcacatagg ctattcccta
      541 aactgcagtt tgaggatttt attgacagag ttgaatacct gggaagtaaa aaggaagttc
      601 agacctgttt aaaacgaatt cnac
//
