LOCUS       sequence-286752-        1226 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3403
FEATURES             Location/Qualifiers
     source          1..1226
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1058..1082)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1090..1131
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc cagagtggcc accttgctta
       61 tctgtggccc cggccctggt gatcaccatg gcagctggga agggagcccc gttgagccca
      121 tcggctgaaa acagatggcg acttagcgaa cctgagctgg gccggggctg caagccagtg
      181 ctgctcgaga agacgaaccg cctgggccct gaggctgctg tgggcagggc gggccgggat
      241 gtgggcagtg cggagctggc actgttggta gccccaggca agccccgacc tggcaagccg
      301 ctgcccccga agacacgtgg agagcagagg cagagcgcct tcacggagct gccgaggatg
      361 aaggaccggc aggtggatgc tcaggcccag gagagggagc acgatgaccc cacaggccaa
      421 cctggtgccc cacagctgac ccagaacatc cccagaggcc cagctggcag caaagtcttc
      481 tctgtgtggc ccagcggagc acgaagtgag caaagaagcg cctttagcaa accaaccaag
      541 cgaccagcag agaggcctga gctaacctca gtcttccctg caggggaatc tgcagatgcc
      601 ctgggggagc tgtctggact cctcaacact acagacctcg cttgttgggg tcgactttca
      661 actcccaagc ttttggttgg tgatttgtgg aacttgcaag cattgccaca gaatgctcca
      721 ctctgtagca cttttctggg tgcccctaca ctgtggctgg agcataccca ggcccaggtg
      781 cccccaccct catcatcctc caccacttcg tgggccctcc tgccacccac cctcacctcc
      841 ctgggcttgt ccacccagaa ctggtgtgca aagtgcaacc tgtcctttcg cctaacgtcc
      901 gacctggtct ttcacatgcg atcccaccac aaaaaggagc atgcggggcc tgacccacat
      961 tctcagaagc ggagagaaga ggcccttgcc tgccctgtgt gccaggagca cttccgggag
     1021 cgccaccacc tctcccggca catgacttct cacagctacc caactttctt gtacaaagtg
     1081 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     1141 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1201 gatttgtcgg ggttgttgca catcca
//
