LOCUS       sequence-286755-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3404
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(794..818)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             826..867
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc agttgccccc cgcgctgtgc
       61 gcccgcctcg ctgcggggcc cggggcggcg gagccactgc ctgtagagcg ggatcccgcg
      121 gctggggccg cgccctttcg cttcgttgcg cgccccgtgc gcttcccgcg ggagcaccag
      181 ttcttcgagg atggggacgt gcagcgccac ctctacctcc aggacgtgat catgcaggtg
      241 gccgacgtgc ctgagaagcc cagggtgccc gcgtttgcct gccaggtggc cggctgctgc
      301 caggtgttcg atgccctgga cgactacgag caccactacc acacgctgca cggaaatgtt
      361 tgctcctttt gcaagcgggc cttcccttcc ggacacctgc tggacgccca catcctggag
      421 tggcacgatt cgctcttcca gatcctgtct gagaggcagg acatgtatca gtgcttggta
      481 gaaggctgca cagagaagtt caagaccagc agagaccgga aggatcacat ggtgaggatg
      541 cacctgtacc ccgcggactt ccggtttgat aagccaaaga aaagcagaag cccagcctca
      601 gcagaagccc caggggacag tggagagcgg tcagaagggg aggccatgga aatctgctct
      661 gagcctgtgg cagcctcccc tgcaccggca ggtgagaggc ggatctacag acatagaata
      721 ccctctacca tctgctttgg tcagggtgcc gctcgaggat ttaaaagcaa caagaagaaa
      781 accaaacaat gctgcccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
      841 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
      901 tcgatttctt ggctttatat atcttgtgga aaggacgatt ctcacttacc cacgctgaca
      961 cc
//
