LOCUS       sequence-286758-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3405
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(788..812)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             820..861
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga atgtgcggag ggtggaaagc
       61 atttcggctc agctggagga ggccagctct acaggcggtt tcctgtacgc tcagaacagc
      121 accaagcgca gcattaaaga gcggctcatg aagctcttgc cctgctcagc tgccaaaacg
      181 tcgtctcctg ctattcaaaa cagcgtggaa gatgaactgg agatggccac cgtcaggcat
      241 cggcccgaag cccttgagct tctggaagcc cagagcaaat ttaccaagaa agagcttcag
      301 atcctttaca gaggatttaa gaacgaatgc cccagtggtg ttgttaatga agaaaccttc
      361 aaagagattt actcgcagtt ctttccacag ggagactcta caacatatgc acattttctg
      421 ttcaatgcat ttgatacaga ccacaatgga gctgtgagtt tcgaggattt catcaaaggt
      481 ctttccattt tgctccgggg gacagtacaa gaaaaactca attgggcatt taatctgtat
      541 gacataaata aagatggcta catcactaaa gaggaaatgc ttgatataat gaaagcaata
      601 tacgatatga tgggtaaatg tacatatcct gtcctcaaag aagatgctcc cagacaacac
      661 gttgaaacat tttttcagaa aatggacaaa aataaagatg gggttgttac catagatgag
      721 ttcattgaaa gctgccaaaa agatgaaaac ataatgcgct ccatgcagct ctttgaaaat
      781 gtgatttacc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
      841 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
      901 tcttggcttt atatatcttg tggaaaggac gagatgacta ttttgacttt ctttct
//
