LOCUS       sequence-28676-e         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTER_MDC1 (422-2)
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(209..226)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    215..439
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(572..591)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttgggatct tataagttcc ctatcagtga tagagatccc ccaacatgca gagattgaaa
       61 ttcaagagat ttcaatctct gcatgttgtt tttggaaaag cttggtaccg agctcggatc
      121 cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc gctcgagtct
      181 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
      241 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
      301 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
      361 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
      421 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
      481 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      541 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
      601 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
      661 tttaggtgct ttacggactc gccagagacc ggtgtcgccc ctagatggaa taataatatt
      721 tactttatgt cgtaggag
//
