LOCUS       sequence-286764-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3407
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(863..887)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             895..936
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctgtggaatc tggagtgatt
       61 tcaaccctga tacctcagga tcctccggaa caagaactaa tactagtgaa agtagaagat
      121 aacttttcct gggatgagaa atttaagcag aatgggagta ctcaatcctg ccaagaattg
      181 tttcgtcagc aattcagaaa attttgctac caggagacac ctgggccccg ggaggctctg
      241 agccgactcc aggaactttg ctatcagtgg ctaatgccag agttgcacac aaaggagcag
      301 atcttagaac tgctggtact ggagcagttc ctgagcattc tgcctgagga gctgcagatc
      361 tgggttcagc aacataatcc agaaagcggc gaggaagctg tgaccctgtt ggaggattta
      421 gagagggagt ttgatgaccc agggcagcag gtcccagcta gtccacaggg accagcagtg
      481 ccatggaagg atttaacatg tctcagagca tcccaagagt caacagacat ccacctccag
      541 cccttaaaga cacagctgaa atcctggaaa ccatgccttt cccctaaaag tgattgtgag
      601 aacagtgaaa cagcaacaaa agagggcatc tcagaagaaa aatcacaggg actccctcag
      661 gaaccttcat ttcgaggaat taagttgtcc agacctccca aagcttcttc agctatccgt
      721 tgggaatgtg tttctccagg aagttttccc ggcgatatca tagctgctga ggctacacat
      781 tcaacaattt cttgctttgc catcaacact ttgccagcca ccatcctgcc atctaaaaat
      841 gtgaatagaa agtatttttc ctgcccaact ttcttgtaca aagtggttga tatcggtaag
      901 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
      961 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgatca tctctctttc
     1021 tatccgtagt c
//
