LOCUS       sequence-286767-        1436 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3408
FEATURES             Location/Qualifiers
     source          1..1436
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1268..1292)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1300..1341
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggagactag accacagagc
       61 aaggattcaa cttcagtgca agatttttcc aaagcagaat catgcaaagt tgcaataata
      121 gacagactga cacggaatag tgtctatgac tctaacttgg aagcagccct tgaatgtgaa
      181 aattggttag agaatcagca aggaaatcag gagagacatt tgagagaaat gttcactcac
      241 atgaattcac tctctgagga aacagaccat aagcatgatg tatactggaa aagcttcaat
      301 cagaaatctg tccttatcac tgaagacaga gttcccaaag gatcttatgc cttccataca
      361 cttgaaaaaa gcttgaaaca aaaatcaaac ttaatgaaaa agcagagaac ttataaagag
      421 aaaaaacctc ataaatgtaa tgattgtggt gaactcttca cctaccattc agtgcttatt
      481 cgacaccaga gagtccatac tggagagaaa ccctatacct gcaatgaatg tgggaaatct
      541 tttagccaca gagctaattt aactaaacac cagagaactc atactagaat tctctttgaa
      601 tgcagtgaat gcaagaaaac cttcacagaa agctcatccc ttgcaacaca tcagagaatt
      661 cacgttggag agagacctta tgaatgcaat gaatgtggga aaggttttaa tcgaagtaca
      721 catcttgtgc agcatcagtt gattcatact ggagtgaagc cttatgaatg taatgaatgt
      781 gataaagctt ttattcattc atcagcactc attaaacatc aaagaactca tactggagag
      841 aaaccttata aatgtcaaga atgtgggaaa gcctttagcc attgctcatc cctaactaag
      901 catcagagag ttcatactgg agaaaagcca tatgaatgca gtgaatgtgg aaaaactttt
      961 agtcagagca cacatcttgt tcaacatcag agaattcaca ctggagagaa accctatgag
     1021 tgtaatgaat gtgggaaaac cttcagccgg agctccaatt ttgctaaaca tcaaagaatt
     1081 catattggaa agaaaccgta caaatgtagc gagtgtggaa aagccttcat tcattcatca
     1141 gctctcattc aacatcagag aactcatacc ggagagaaac cctttagatg taatgagtgt
     1201 gggaaaagct ttaagtgcag ttcatctctc atcagacatc aaagagttca cactgaagag
     1261 caacccttgc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1321 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1381 tcttggcttt atatatcttg tggaaaggac gacgggctca atccgagcgg caacga
//
