LOCUS       sequence-286770-        1526 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3409
FEATURES             Location/Qualifiers
     source          1..1526
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1358..1382)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1390..1431
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgctccaaac aacttggcct
       61 caggagtcag tgacctttga ggatgtggct gtttacttca cccagaatca atgggccagc
      121 ctcgaccctg cgcagagggc cctgtacggg gaggtgatgc tggagaatta tgcaaatgtg
      181 gcttctctgg tagcgtttcc attccccaaa cctgctctga tctcccacct ggagagaggg
      241 gaagcaccat ggggcccaga tccctgggac accgagattc tgagaggcat cagtcaaggt
      301 ggtgagtcct ggatcaaaaa tgaagggcta gttataaagc aggaagcctc tgaagaaaca
      361 gagttgcaca gaatgccagt aggaggactt ctcaggaacg tttctcagca ctttgatttt
      421 aaaaggaagg cactgaagca gactttcaat ctaaatccaa atctgatact tcgaggtgga
      481 atgaagttct atgaatgtaa agaatgtggg aaaatcttcc gatataactc aaagcttatt
      541 cggcatcaga tgagtcatac tggggaaaag ccctttaagt gtaaggagtg tggcaaagct
      601 ttcaagtcca gctatgattg tattgtacat gagaaaaacc acattggaga agggccctat
      661 gaatgtaagg agtgtggcaa aggtttgagt tccaacacag ccttgactca acatcagagg
      721 atccacactg gagagaaacc ctatgaatgt aaagagtgtg gaaaggcttt ccgtaggagt
      781 gcggcatacc tgcagcatca gagattacac acgggagaga aactctataa atgtaaggaa
      841 tgttggaaag ctttcggttg taggtcactt tttattgtcc atcagagaat tcatactggg
      901 gagaaacctt atcaatgtaa ggagtgtggc aaagccttca cccagaaaat agcctccatt
      961 cagcatcaga gagttcacac tggggagaag ccttatgaat gtaaggtgtg tgggaaagcc
     1021 ttcaaatggt atggaagttt tgttcagcat cagaaattgc accctgtgga gaagaagcca
     1081 gtcaaggtcc ttgggccatc cctggtcagt ccccagtgct cctctccagc catacctcct
     1141 gttcttctcc agggatcctg ttctgcttca gccgtagctg tgccttcact gacctttcca
     1201 catgctgtgc tcattcctac ctctgggaat tttttcatgc tgctgcctac atctggaata
     1261 ccttcttcat ctgcccaaat agtgcgtgtc ttccagggtc ttactcccac tgtgaaacct
     1321 tccccagtta ttctcacccc ttcttctcac tcctcattgc caactttctt gtacaaagtg
     1381 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     1441 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1501 gaatagacgt ttacattagt tcgcgc
//
