LOCUS       sequence-286773-        1841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3410
FEATURES             Location/Qualifiers
     source          1..1841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1673..1697)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1705..1746
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctagagaacc gagaaaaaac
       61 gcagccctgg acgcccagtc tgcagaagac cagacggggc tcctgaccgt gaaggtggag
      121 aaggaggaag cctccgcctt gacggcggag gtgagagcac cctgcagccc tgctcggggc
      181 cccgaacgct cccgccagcg cttccgaggc ttccgctacc cggaggctgc gggcccccgc
      241 gaggcgttga gccggctccg agaactctgc ggacaatggc tgcagcctga gatgcacagc
      301 aaggagcaga tcctggagct gctggtgctg gagcagttcc tgaccatcct gccggggaat
      361 ctgcagagct gggtgcggga gcagcatcca gagagcgggg aggaggtggt ggtgctattg
      421 gagtatttgg agaggcagct ggatgagccg gcgccgcagg ttcccgttgg tgaccagggg
      481 caagaactgc tctgttgcaa gatggcacta ttgacacaaa ctcaagggtc tcaaagtagc
      541 cagtgccagc caatgaaggc tctgttcaag catgaatctc tgggatccca gcccttacac
      601 gatagagttc tccaggttcc tgggcttgcc cagggagggt gctgcagaga agatgcaatg
      661 gtagcttcca ggctcactcc agggtcccag ggtttgctga aaatggaaga tgtggccctg
      721 accctcactc ctgggtggac acagctggat tcatctcagg tgaacctcta cagagatgaa
      781 aagcaggaga accatagcag cctggtctcc cttggtggtg aaatacagac taagagcagg
      841 gacttgcctc cagtcaagaa gcttccagaa aaggagcatg ggaagatatg ccacctgagg
      901 gaagacattg cccagattcc tacacatgca gaagctggtg aacaggaggg caggttacaa
      961 agaaagcaga aaaatgccat agggagtagg cgacattatt gccatgaatg tggaaagagt
     1021 tttgctcaaa gttcaggcct gactaaacac aggagaatcc acactggtga gaaaccctat
     1081 gaatgtgaag actgtggaaa gaccttcatt gggagctctg cccttgtcat tcatcagaga
     1141 gtccacactg gtgagaagcc atatgagtgt gaagaatgtg gtaaggtctt cagtcacagc
     1201 tcaaacctta tcaaacacca gagaacccac actggggaga agccctatga gtgtgatgac
     1261 tgtgggaaga ccttcagcca gagctgcagc ctccttgaac atcacaaaat tcatactggg
     1321 gagaagccat accagtgcaa tatgtgtggc aaagccttta ggcggaattc acatctcctg
     1381 agacatcaga ggattcatgg tgataaaaat gttcagaatc ctgagcacgg ggagtcctgg
     1441 gaaagtcagg gtaggacgga aagccagtgg gaaaatactg aggctcccgt gtcttataaa
     1501 tgtaatgagt gtgagagaag tttcacacgg aatagaagtc ttattgaaca tcagaaaatc
     1561 cacactggtg agaaacccta tcagtgtgac acatgtggaa aaggtttcac tcgaacttca
     1621 taccttgttc aacatcagag aagccatgta gggaaaaaaa ctctttcaca gtgcccaact
     1681 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     1741 tctacgtagt aatgaactag tccggaactt gaaagtattt cgatttcttg gctttatata
     1801 tcttgtggaa aggacgagcc ttcaatggaa ttgcatcaat c
//
