LOCUS       sequence-286779-        1835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the EGR1 transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3412
FEATURES             Location/Qualifiers
     source          1..1835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1667..1691)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1699..1740
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ccgcggccaa ggccgagatg
       61 cagctgatgt ccccgctgca gatctctgac ccgttcggat cctttcctca ctcgcccacc
      121 atggacaact accctaagct ggaggagatg atgctgctga gcaacggggc tccccagttc
      181 ctcggcgccg ccggggcccc agagggcagc ggcagcaaca gcagcagcag cagcagcggg
      241 ggcggtggag gcggcggggg cggcagcaac agcagcagca gcagcagcac cttcaaccct
      301 caggcggaca cgggcgagca gccctacgag cacctgaccg cagagtcttt tcctgacatc
      361 tctctgaaca acgagaaggt gctggtggag accagttacc ccagccaaac cactcgactg
      421 ccccccatca cctatactgg ccgcttttcc ctggagcctg cacccaacag tggcaacacc
      481 ttgtggcccg agcccctctt cagcttggtc agtggcctag tgagcatgac caacccaccg
      541 gcctcctcgt cctcagcacc atctccagcg gcctcctccg cctccgcctc ccagagccca
      601 cccctgagct gcgcagtgcc atccaacgac agcagtccca tttactcagc ggcacccacc
      661 ttccccacgc cgaacactga cattttccct gagccacaaa gccaggcctt cccgggctcg
      721 gcagggacag cgctccagta cccgcctcct gcctaccctg ccgccaaggg tggcttccag
      781 gttcccatga tccccgacta cctgtttcca cagcagcagg gggatctggg cctgggcacc
      841 ccagaccaga agcccttcca gggcctggag agccgcaccc agcagccttc gctaacccct
      901 ctgtctacta ttaaggcctt tgccactcag tcgggctccc aggacctgaa ggccctcaat
      961 accagctacc agtcccagct catcaaaccc agccgcatgc gcaagtaccc caaccggccc
     1021 agcaagacgc ccccccacga acgcccttac gcttgcccag tggagtcctg tgatcgccgc
     1081 ttctcccgct ccgacgagct cacccgccac atccgcatcc acacaggcca gaagcccttc
     1141 cagtgccgca tctgcatgcg caacttcagc cgcagcgacc acctcaccac ccacatccgc
     1201 acccacacag gcgaaaagcc cttcgcctgc gacatctgtg gaagaaagtt tgccaggagc
     1261 gatgaacgca agaggcatac caagatccac ttgcggcaga aggacaagaa agcagacaaa
     1321 agtgttgtgg cctcttcggc cacctcctct ctctcttcct acccgtcccc ggttgctacc
     1381 tcttacccgt ccccggttac tacctcttat ccatccccgg ccaccacctc atacccatcc
     1441 cctgtgccca cctccttctc ctctcccggc tcctcgacct acccatcccc tgtgcacagt
     1501 ggcttcccct ccccgtcggt ggccaccacg tactcctctg ttccccctgc tttcccggcc
     1561 caggtcagca gcttcccttc ctcagctgtc accaactcct tcagcgcctc cacagggctt
     1621 tcggacatga cagcaacctt ttctcccagg acaattgaaa tttgctaccc aactttcttg
     1681 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     1741 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1801 ggaaaggacg agttttggct acaattcgcg caagc
//
