LOCUS       sequence-28678-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti X1 Zeo/pTER shMDC1 (673-1)
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    23..41
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(173..197)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(281..301)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(699..710)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cttggattgg gatcttataa gttccctatc agtgatagag atcccccaac atgcagagat
       61 tgaaattcaa gagatttcaa tctctgcatg ttgtttttgg aaaagcttgg taccgagctc
      121 ggatccacta gtccagtgtg gtggaattcg cggccgcact cgagatatct agacccagct
      181 ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgacaat caacctctgg
      241 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
      301 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
      361 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
      421 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
      481 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
      541 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
      601 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
      661 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
      721 ttccttcccg cggcctgctg ccggctctgc gcctcttcg
//
