LOCUS       sequence-286782-        1802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3413
FEATURES             Location/Qualifiers
     source          1..1802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1634..1658)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1666..1707
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cccttcgatc agtgatgttc
       61 agtgatgtat ccatagactt ctctccagaa gagtgggaat acctggactt ggaacaaaag
      121 gacttgtaca gagatgtcat gttggagaac tacagcaact tggtctcact gggatgcttc
      181 atttctaaac cagatgtgat ttcctcattg gagcaaggaa aagagccttg gaaagttgtg
      241 aggaaaggaa gaagacaata tccagatttg gagaccaagt atgagaccaa gaagttatct
      301 ttagaaaatg acatttatga aataaattta tcccagtgga agataatgga aagaattgaa
      361 aaccatggcc ttaagggtct cattttaaaa aatgattggg aatccacagg aaaaattgaa
      421 ggacaggaga gacctcaaga aggatacttc agtagtgtga aaatgccatc tgaaaaggtg
      481 tcctcttacc agaaacgcac atctgttact ccacatcaga gacttcattt tgttgataaa
      541 ccctatgaat gtaaggaatg tgggaaggcg ttcagagtgc gccaacagct tacttttcat
      601 cacagaattc atactggtga aaaaccgtat gaatgtaagg aatgtgggat ggccttcaga
      661 cagactgcac accttactcg acatcagaga cttcattctg gtgaaaaact ctatgaatgt
      721 aaggaatgtg gggaagcttt catatgtggt gcagatctta gagtacatca gaaaatgcat
      781 attggtgaga agccctatga atgtaaagaa tgtgggaagg cttttagggt acgaggacaa
      841 cttactctgc atcagaggat tcatactggt gagaaaccct atgtgtgtaa agagtgtgga
      901 aaagccttta gacagtacgc acacctgact cggcatcaga agcttaatag tgctgacagg
      961 ctctatgaat gcaaagaatg tgggaaggcc tttttgtgtg gctctggtct tagagtacat
     1021 cacaaacttc atactggtga gaaaccctat gaatgtaagg aatgcgggaa ggcctttaga
     1081 gtgcgacaac aactaacact ccatcagaga attcatactg gtgagaaacc ctatgaatgt
     1141 aaggaatgtg gaaagacctt tagccgtggc tatcatctta ttctccatca cagaattcat
     1201 actggtgaaa aaccttacga atgtaaggaa tgctggaaag cctttagtcg ctactcacaa
     1261 cttatttcac atcagagtat tcatattggt gttaagccct atgactgtaa ggaatgcggg
     1321 aaggccttca gactactttc acaactcaca cagcatcaga gtattcatat tggtgagaaa
     1381 ccttataaat gtaaggaatg tggcaaggcc tttagattgc gccaaaaact tactctacat
     1441 cagagcattc atactggcga aaaacccttt gagtgtaagg aatgtaggaa ggcctttaga
     1501 cttaattcat cccttattca acatctgaga attcattctg gtgagaaacc ctatgaatgt
     1561 aaggaatgta agaaggcctt taggcaacat tcacacctta ctcatcatct gaaaattcat
     1621 aatgtaaaaa tctacccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     1681 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     1741 tcgatttctt ggctttatat atcttgtgga aaggacgagc tttttgacga tcaatctagc
     1801 ac
//
