LOCUS       sequence-286785-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3414
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(749..773)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             781..822
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc cagcccgcct tgagacctgc
       61 atctccgacc tcgactgcgc cagcagcagc ggcagtgacc tatccggctt cctcaccgac
      121 gaggaagact gtgccagact ccaacaggca gcctccgctt cggggccgcc cgcgccggcc
      181 cgcaggggcg cgcccaatat ctcccgggcg tctgaggttc caggggcaca ggacgacgag
      241 caggagaggc ggcggcgccg cggccggacg cgggtccgct ccgaggcgct gctgcactcg
      301 ctgcgcagga gccggcgcgt caaggccaac gatcgcgagc gcaaccgcat gcacaacttg
      361 aacgcggccc tggacgcact gcgcagcgtg ctgccctcgt tccccgacga caccaagctc
      421 accaaaatcg agacgctgcg cttcgcctac aactacatct gggctctggc cgagacactg
      481 cgcctggcgg atcaagggct gcccggaggc ggtgcccggg agcgcctcct gccgccgcag
      541 tgcgtcccct gcctgcccgg tcccccaagc cccgccagcg acgcggagtc ctggggctca
      601 ggtgccgccg ccgcctcccc gctctctgac cccagtagcc cagccgcctc cgaagacttc
      661 acctaccgcc ccggcgaccc tgttttctcc ttcccaagcc tgcccaaaga cttgctccac
      721 acaacgccct gtttcattcc ttaccactac ccaactttct tgtacaaagt ggttgatatc
      781 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
      841 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgactcacct
      901 atgtaagatc aattttc
//
