LOCUS       sequence-286788-        2279 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3415
FEATURES             Location/Qualifiers
     source          1..2279
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2111..2135)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2143..2184
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggaaaaaat tctccaaatg
       61 gctgaaggta ttgatattgg ggagatgcct tcatatgatc tggtgctgtc caaaccttcc
      121 aaaggtcaaa aacgccacct ctcaacatgt gatggtcaaa atcctcctaa aaagcaagcc
      181 ggttccaaat tccatgcgag acctcgtttt gagcctgtac attttgtagc tagtagttca
      241 aaagatgaaa gacaggaaga tccttatggc cctcaaacaa aagaggtaaa tgaacaaaca
      301 cattttgcca gcatgccaag agacatctac caagattata ctcaagactc tttcagtata
      361 caagatggga attctcagta ttgtgattca tcaggattca ttctcacaaa agaccagcct
      421 gtaacagcca acatgtattt tgacagtggg aaccctgccc caagcaccac atcacagcag
      481 gcaaactctc agtcaactcc tgagccttca ccatcacaga catttcccga gtctgtggta
      541 gccgagaagc agtattttat tgaaaaatta acggcgacaa tctggaagaa cctttctaat
      601 ccagaaatga cttctggatc tgataaaatt aattatacat atatgttaac tcgttgtatt
      661 caggcgtgta agacaaatcc tgagtatata tatgctcctt taaaggaaat tcctcctgcc
      721 gacatcccca aaaataaaaa acttctaact gatggctatg cttgtgaagt tagatgccaa
      781 aatatctact taactacagg ttatgctggc agcaagaatg ggtccaggga tcgagctaca
      841 gagctagctg taaaactctt gcagaaacgt attgaagtta gagttgtccg gcggaaattc
      901 aagcatacat ttggagagga cctcgtggtg tgtcagattg gcatgtcctc ctatgaattt
      961 cctccagctc tgaagccacc agaagacctg gtggtgctgg gtaaagatgc ttccgggcag
     1021 ccaattttta atgcttctgc caaacactgg accaattttg tcattacaga aaatgcaaat
     1081 gatgcaattg gtatccttaa caattctgcc tcattcaaca agatgtcaat tgaatacaaa
     1141 tatgagatga tgccaaatcg cacatggcgt tgtcgagtgt ttttacaaga tcactgctta
     1201 gctgaaggtt atggaaccaa gaaaacaagt aaacatgcag ctgccgacga ggctttgaaa
     1261 attcttcaaa aaacacagcc cacttatcca tctgtcaaaa gttcacaatg ccatacaggc
     1321 tcttcaccca gaggatctgg aaagaagaaa gatataaagg atcttgtagt ttatgagaat
     1381 tcttcaaatc ccgtgtgcac gctgaacgac acagctcagt ttaaccgaat gacagttgag
     1441 tatgtctatg aaaggatgac aggcctccgc tggaaatgca aagtgattct agagagtgaa
     1501 gtaattgcag aagcagttgg ggtgaagaaa actgtcaaat atgaagctgc tggggaagct
     1561 gtgaaaaccc tcaaaaagac ccagccaact gtcattaaca acttgaagaa aggagctgtt
     1621 gaagatgtga tttcaagaaa tgaaattcag ggccgctcag cagaggaggc ttacaaacag
     1681 caaatcaaag aagataatat tggaaatcag ctgctgagaa agatgggttg gactggtggt
     1741 ggtttaggta aatctggtga gggcatacgg gagcctatct cagtgaaaga gcagcataag
     1801 cgggaagggc ttggtctgga tgtagagagg gtgaataaaa ttgccaagag agatattgaa
     1861 cagatcatca gaaactacgc ccgctccgag agccacacag atttgacttt ctctagagag
     1921 ctgactaatg atgaacggaa gcaaatacat cagattgccc agaagtatgg tcttaagagt
     1981 aagtctcatg gggtgggcca tgataggtac ctagtggtag gtagaaaaag acggaaggaa
     2041 gacctactag atcagctcaa acaggaaggc caagtgggcc attacgagct tgttatgcct
     2101 caagcaaatt tgccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     2161 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     2221 atttcttggc tttatatatc ttgtggaaag gacgaagtct tgggtaagcc ttcctaaaa
//
