LOCUS       sequence-28679-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti X2 Zeo/pTER shMDC1 (764-1)
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..44
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(176..200)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(336..704)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="KLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVT
                     LFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREF
                     ALRDPAGNCVHFVAEEQD"
ORIGIN
        1 tcttngattt gggaatctta taagttccct atcagtgata gagatccccc aacatgcaga
       61 gattgaaatt caagagattt caatctctgc atgttgtttt tggaaaagct tggtaccgag
      121 ctcggatcca ctagtccagt gtggtggaat tcgcggccgc actcgagata tctagaccca
      181 gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagccctt
      241 ccagtccccc cttttctttt aaaaagtggc taagatctac agctgccttg taagtcattg
      301 gtcttaaagg taccatttaa atctcgtagc acgtgtcagt cctgctcctc ggccacgaag
      361 tgcacgcagt tgccggccgg gtcgcgcagg gcgaactccc gcccccacgg ctgctcgccg
      421 atctcggtca tggccggccc ggaggcgtcc cggaagttcg tggacacgac ctccgaccac
      481 tcggcgtaca gctcgtccag gccgcgcacc cacacccagg ccagggtgtt gtccggcacc
      541 acctggtcct ggaccgcgct gatgaacagg gtcacgtcgt cccggaccac accggcgaag
      601 tcgtcctcca cgaagtcccg ggagaacccg agccggtcgg tccagaactc gaccgctccg
      661 gcgacgtcgc gcgcggtgag caccggaacg gcactggtca acttgggcca tggtttagtt
      721 cctcaccttg tcgtattata ctatgccgat atactatgcc gatgataatt gtcacagggg
      781 agcttttgca aagcctaggc tcaaaagctc t
//
