LOCUS       sequence-286791-        1499 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3416
FEATURES             Location/Qualifiers
     source          1..1499
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1331..1355)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1363..1404
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt tatcagatga gttagaatcc
       61 aaaccagagc tcctggtaca gtttgttcag aatacgtcca tcccattggg acaggggctt
      121 gtagaatcag aagctaaaga tattacttgc ttgtccctcc ttcccgtgac tgaagcctca
      181 gaatgcagtc ggctaatgtt accagatgat actacaaatc attctaactc ctccaaggag
      241 gtcccttcct cagctgtttt gagaagcctt cgggtgaatg tgggtccaga cggagaggag
      301 acgagagctc agactgtaca gaaatccccg gagtttttgt ccacttcaga gtcttctagc
      361 ttgttgcaag atctacagcc aagtgatagc acttctttta ttcttcttaa cctaacaaga
      421 gcaggtctgg gctcttcagc tgagcactta gtgtttgtac aggatgaggc agaagattca
      481 gggaatgatt tcctctccag tgagagcaca gacagtagca ttccatggtt cctccgggtt
      541 caggagttgg cccatgacag tttgattgct gctactcgtg cacaactggc aaagaatgca
      601 aaaaccagca gcaatggaga aaatgtccac cttggttctg gtgatgggca gtcaaaagat
      661 tctgggcccc ttcctcaagt ggaaaagaag ctcaagtgta cagttgaagg ttgtgaccgg
      721 acatttgtat ggccagctca ctttaaatac cacctcaaga ctcatcgaaa tgaccgctcc
      781 ttcatctgtc ctgcagaagg ttgtgggaaa agcttctatg tgctgcagag gctgaaggtg
      841 cacatgagga cccacaatgg agagaagccc tttatgtgcc atgagtctgg ctgtggtaag
      901 cagtttacta cagctggaaa cctgaagaac caccggcgca tccacacagg agagaaacct
      961 ttcctttgtg aagcccaagg atgtggccgt tcctttgctg agtattctag cctccgaaaa
     1021 catctggtgg ttcactcagg agagaagcct catcagtgcc aagtctgtgg gaagaccttc
     1081 tctcagagtg gaagcaggaa tgtgcatatg agaaagcatc acctgcagct gggagcagct
     1141 gggagtcaag agcaggagca aactgaggtg cttgctgaag gatccccacg ttccctgtct
     1201 tcagtgcctg atgtgacaca tcacctggtg accatgcagt cagggaggca atcatatgaa
     1261 gtttctgtct taactgcagt aaatccacaa gagttactaa accaaggaga tttaactgaa
     1321 agacggacat gcccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     1381 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1441 atttcttggc tttatatatc ttgtggaaag gacgagacct gtcagatcct ccccacgtc
//
