LOCUS       sequence-286794-        1571 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3417
FEATURES             Location/Qualifiers
     source          1..1571
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1403..1427)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1435..1476
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc cgcagctgaa cggcggtgga
       61 ggggatgacc taggcgccaa cgacgaactg atttccttca aagacgaggg cgaacaggag
      121 gagaagagct ccgaaaactc ctcggcagag agggatttag ctgatgtcaa atcgtctcta
      181 gtcaatgaat cagaaacgaa tcaaaacagc tcctccgatt ccgaggcgga aagacggcct
      241 ccgcctcgct ccgaaagttt ccgagacaaa tcccgggaaa gtttggaaga agcggccaag
      301 aggcaagatg gagggctctt taaggggcca ccgtatcccg gctacccctt catcatgatc
      361 cccgacctga cgagccccta cctccccaac ggatcgctct cgcccaccgc ccgaacctat
      421 ctccagatga aatggccact gcttgatgtc caggcaggga gcctccagag tagacaagcc
      481 ctcaaggatg cccggtcccc atcaccggca cacattgtct ctaacaaagt gccagtggtg
      541 cagcaccctc accatgtcca ccccctcacg cctcttatca cgtacagcaa tgaacacttc
      601 acgccgggaa acccacctcc acacttacca gccgacgtag accccaaaac aggaatccca
      661 cggcctccgc accctccaga tatatccccg tattacccac tatcgcctgg caccgtagga
      721 caaatccccc atccgctagg atggcaaggt caaccagtgt acccaatcac gacaggagga
      781 ttcagacacc cctaccccac agctctgacc gtcaatgctt ccatgtccag gttccctccc
      841 catatggtcc caccacatca tacgctacac acgacgggca ttccgcatcc ggccatagtc
      901 acaccaacag tcaaacagga atcgtcccag agtgatgtcg gctcactcca tagttcaaag
      961 catcaggact ccaaaaagga agaagaaaag aagaagcccc acataaagaa acctcttaat
     1021 gcattcatgt tgtatatgaa ggaaatgaga gcaaaggtcg tagctgagtg cacgttgaaa
     1081 gaaagcgcgg ccatcaacca gatccttggg cggaggtggc atgcactgtc cagagaagag
     1141 caagcgaaat actacgagct ggcccggaag gagcgacagc ttcatatgca actgtacccc
     1201 ggctggtccg cgcgggataa ctatggaaag aagaagaaga ggaaaaggga caagcagccg
     1261 ggagagacca atgaacacag cgaatgtttc ctaaatcctt gcctttcact tcctccgatt
     1321 acaggagaaa aaaaaagtgc gttcgctaca tacaaggtga aggcagctgc ctcagcccat
     1381 cctcttcaga tggaagctta ctacccaact ttcttgtaca aagtggttga tatcggtaag
     1441 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1501 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagcc tcgcggcgtg
     1561 acgatttctt c
//
