LOCUS       sequence-286797-        2021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3418
FEATURES             Location/Qualifiers
     source          1..2021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1853..1877)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1885..1926
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga tggacttgga gctgccgccg
       61 ccgggactcc cgtcccagca ggacatggat ttgattgaca tactttggag gcaagatata
      121 gatcttggag taagtcgaga agtatttgac ttcagtcagc gacggaaaga gtatgagctg
      181 gaaaaacaga aaaaacttga aaaggaaaga caagaacaac tccaaaagga gcaagagaaa
      241 gcctttttcg ctcagttaca actagatgaa gagacaggtg aatttctccc aattcagcca
      301 gcccagcaca tccagtcaga aaccagtgga tctgccaact actcccaggt tgcccacatt
      361 cccaaatcag atgctttgta ctttgatgac tgcatgcagc ttttggcgca gacattcccg
      421 tttgtagatg acaatgaggt ttcttcggct acgtttcagt cacttgttcc tgatattccc
      481 ggtcacatcg agagcccagt cttcattgct actaatcagg ctcagtcacc tgaaacttct
      541 gttgctcagg tagcccctgt tgatttagac ggtatgcaac aggacattga gcaagtttgg
      601 gaggagctat tatccattcc tgagttacag tgtcttaata ttgaaaatga caagctggtt
      661 gagactacca tggttccaag tccagaagcc aaactgacag aagttgacaa ttatcatttt
      721 tactcatcta taccctcaat ggaaaaagaa gtaggtaact gtagtccaca ttttcttaat
      781 gcttttgagg attccttcag cagcatcctc tccacagaag accccaacca gttgacagtg
      841 aactcattaa attcagatgc cacagtcaac acagattttg gtgatgaatt ttattctgct
      901 ttcatagctg agcccagtat cagcaacagc atgccctcac ctgctacttt aagccattca
      961 ctctctgaac ttctaaatgg gcccattgat gtttctgatc tatcactttg caaagctttc
     1021 aaccaaaacc accctgaaag cacagcagaa ttcaatgatt ctgactccgg catttcacta
     1081 aacacaagtc ccagtgtggc atcaccagaa cactcagtgg aatcttccag ctatggagac
     1141 acactacttg gcctcagtga ttctgaagtg gaagagctag atagtgcccc tggaagtgtc
     1201 aaacagaatg gtcctaaaac accagtacat tcttctgggg atatggtaca acccttgtca
     1261 ccatctcagg ggcagagcac tcacgtgcat gatgcccaat gtgagaacac accagagaaa
     1321 gaattgcctg taagtcctgg tcatcggaaa accccattca caaaagacaa acattcaagc
     1381 cgcttggagg ctcatctcac aagagatgaa cttagggcaa aagctctcca tatcccattc
     1441 cctgtagaaa aaatcattaa cctccctgtt gttgacttca acgaaatgat gtccaaagag
     1501 cagttcaatg aagctcaact tgcattaatt cgggatatac gtaggagggg taagaataaa
     1561 gtggctgctc agaattgcag aaaaagaaaa ctggaaaata tagtagaact agagcaagat
     1621 ttagatcatt tgaaagatga aaaagaaaaa ttgctcaaag aaaaaggaga aaatgacaaa
     1681 agccttcacc tactgaaaaa acaactcagc accttatatc tcgaagtttt cagcatgcta
     1741 cgtgatgaag atggaaaacc ttattctcct agtgaatact ccctgcagca aacaagagat
     1801 ggcaatgttt tccttgttcc caaaagtaag aagccagatg ttaagaaaaa ctacccaact
     1861 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     1921 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1981 tcttgtggaa aggacgagtg aatttatgtc tctatttatt g
//
