LOCUS       sequence-28680-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti X2 Neo/pTER shMDC1 (w24-2)
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(170..194)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(439..458)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gattgggaat cttataagtt ccctatcagt gatagagatc ccccaacatg cagagattga
       61 aattcaagag atttcaatct ctgcatgttg tttttggaaa agcttggtac cgagctcgga
      121 tccactagtc cagtgtggtg gaattcgcgg ccgcactcga gatatctaga cccagctttc
      181 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgagtctagc ccttccagtc
      241 cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta
      301 aaggtaccgg gccctcgacg gatcccctca gaagaactcg tcaagaaggc gatagaaggc
      361 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
      421 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
      481 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
      541 cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt
      601 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
      661 atcgacaaga ccgnctncat ccgagtacgt gctcgctcga tgcgatgttn cgctnggtgg
      721 tcgaatgggc aggtagccgg atcaagcgta tgcagccccg cattgctcag cntgat
//
