LOCUS       sequence-286800-        1487 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3419
FEATURES             Location/Qualifiers
     source          1..1487
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1319..1343)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1351..1392
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgtcagcctt tgatatgtcc
       61 catgggtttt ttcccaggga accaatctgt ccttttgaag aaaagacaaa gataggaacg
      121 atggtagagg accaccggtc aaattcttac caggattcag tgacgtttga tgatgtggct
      181 gtggagttca ccccagagga gtgggcttta ctggacacaa ctcagaaata cctctacaga
      241 gatgtgatgc tggagaacta catgaacctg gcctctgtgg atttcttttt ctgcttaact
      301 tcagaatggg aaatacaacc tagaaccaaa cggtcatcac ttcagcaggg ttttttgaag
      361 aatcaaatat tcactgggat acaaatgcag acaagaagct acagtggatg gaaactctgt
      421 gagaattgtg gagaggtctt cagtgaacag ttttgcctta agacacacat gagagctcag
      481 aatggaggga acacttttga gggtaattgt tatggaaaag acagcatcag tgtgcacaag
      541 gaagcctcta ttggacaaga actttccaaa tttaatccat gtggaaaagt cttcacctta
      601 actccaggcc ttgctgtgca tcttgaaatt ctcaatggaa gacaacccta caaatgtaag
      661 gaatgtggaa aaggctttaa gtattttgca agccttgata atcacatggg aatccacatt
      721 ggagagaaac tctgtgaatt tcaggaatgt gagagagcca tcacaacttc ctcacactta
      781 aagcagtgtg tagcagttca tactggaaag aaatccgaaa agactaagaa ctgtgggaaa
      841 tccttcacta atttttctca actttctgca catgcaaaaa ctcataaagg agagaagtcc
      901 tttgaatgta aagaatgtgg aagatccttt agaaattcct catcctttaa tgttcacatt
      961 caaattcaca ctggaataaa accacacaaa tgtacggaat gtgggaaagc cttcactaga
     1021 tcaactcacc ttactcaaca tgtaagaact cacactggaa taaaacccta tgaatgtaag
     1081 gaatgtggcc aagccttcac tcagtacacg ggccttgcta tacacatacg aaatcacact
     1141 ggagagaaac cctatcagtg taaggaatgt gggaaagcct tcaatagatc ttcaacgctt
     1201 actcaacata gaagaattca cacaggagag aagccttatg aatgtgttga atgtgggaag
     1261 accttcatta cttcttccca tcgtagtaaa catttgaaaa ctcacagtgg agaaaggttg
     1321 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     1381 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1441 tatatatctt gtggaaagga cgatgatttc aacacttcat tttcagg
//
