LOCUS       sequence-286803-        1310 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3420
FEATURES             Location/Qualifiers
     source          1..1310
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1142..1166)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1174..1215
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ggactcctcc cggcctgcag
       61 accgactgcg aggcgctgct cagccgcttc caggagacgg acagtgtacg cttcgaggac
      121 ttcacggagc tctggagaaa catgaagttc gggactatct tctgtggcag aatgagaaat
      181 ttagaaaaga acatgtttac aaaagaagct ttagctttgg cttggcgata ttttttacct
      241 ccatacacct tccagatcag agttggtgct ttgtatctgc tatatggatt atataatacc
      301 caactgtgtc aaccaaaaca aaagatcaga gttgccctga aggattggga tgaagtttta
      361 aaatttcagc aagatttagt aaatgcacag cattttgatg cagcttatat ttttaggaag
      421 ctacgactag acagagcatt tcactttaca gcaatgccca aattgctgtc atataggatg
      481 aagaaaaaaa ttcaccgagc tgaagttaca gaagaattta aggacccaag tgatcgtgtg
      541 atgaaactta tcacttctga tgtattagag gaaatgctga atgttcatga tcattatcag
      601 aacatgaaac atgtaatttc agttgataag tccaagccag ataaagccct cagcttgata
      661 aaggatgatt tttttgacaa tattaagaac atagttttgg agcatcagca gtggcacaaa
      721 gacagaaaga atccatcctt aaagtcaaaa actaatgatg gagaagaaaa aatggaagga
      781 aattcacaag aaacggagag atgtgaaagg gcagaatcat tagcgaaaat aaaatcaaag
      841 gccttttcag ttgtcataca ggcatccaaa tcaagaaggc atcgtcaagt caaactcgac
      901 tcttctgact ctgattctgc atctggtcaa gggcaagtca aagcaactag gaaaaaagag
      961 aagaaagaaa gattgaaacc agcaggaagg aagatgtctc tcagaaacaa aggcaatgtg
     1021 cagaatatac acaaggaaga taaaccttta agtctgagta tgcctgtaat tacagaagaa
     1081 gaagagaatg aaagtttgag tggaacagag ttcactgcat ccaagaagag gagaaaacac
     1141 tgcccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     1201 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1261 ctttatatat cttgtggaaa ggacgaagac ataacataat caaagtatag
//
