LOCUS       sequence-286806-        1298 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3421
FEATURES             Location/Qualifiers
     source          1..1298
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      617..626
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1130..1154)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1162..1203
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gcgaggtgga ggcggcagcg
       61 ggggctacag cggtccccgc ggcgacggtg cccgcgacgg cggcaggggt ggtagcggtg
      121 gtggtaccgg tgcccgcagg ggagccgcag aaaggcggcg gggcgggcgg cgggggcgga
      181 gccgcctcgg gccccgctgc tgggaccccc tcggcgccgg gctcccgcac ccctggcaat
      241 ccggcgacgg cggtctcggg aacccccgcc cccccggccc ggagtcaggc ggacaagccg
      301 gtgctggcaa tccaagtcct gggcactgtc aaatggttca acgtccggaa tggttacgga
      361 ttcatcaaca ggaatgacac caaggaagat gtctttgttc accagacagc tattaaaaga
      421 aacaacccca ggaagtttct gcgcagcgtt ggagatgggg agactgtgga atttgatgtc
      481 gtggaaggag agaagggcgc agaagccact aatgtaactg ggcctggggg agtacccgtg
      541 aagggcagcc gttatgcccc caaccgacgt aagtcccgcc gattcatccc ccggcctccc
      601 tcagttgccc caccacccat ggtggcagag atcccctcgg cggggacagg acctggcagt
      661 aaaggggagc gggctgaaga ctctgggcaa cggccccgac gatggtgccc cccacccttc
      721 ttctaccgac ggcggtttgt gcgaggcccc cggcctccca accagcagca gcctatagag
      781 ggcactgaca gggtagaacc caaagagaca gccccattgg aggggcacca acagcaggga
      841 gatgagcgag tccccccgcc cagattccgg cccaggtacc gaaggccttt ccgccccagg
      901 ccacgccagc agcctaccac agaaggtggg gatggtgaga ccaagcccag ccaaggtccc
      961 gctgatggtt cccggcctga gccccagcgc ccacgaaacc gcccctactt ccagcggaga
     1021 cggcagcagg cccctggccc ccagcaggcc cctggccccc ggcagcccgc agcccctgag
     1081 acctcagccc ctgtcaacag tggggacccc accaccacca tcctggagtg cccaactttc
     1141 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
     1201 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1261 tgtggaaagg acgacccatg tgatctcgcg attgtgtc
//
