LOCUS       sequence-286809-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3422
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(710..734)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             742..783
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cgccacccgc ggcgcctggc
       61 cgggaccgtg tgggccgtga ggatgaggac ggctgggaga cgcgagggga ccgcaaggcc
      121 cggaagcccc tggtggagaa gaagcggcgc gcgcggatca acgagagcct gcaggagctg
      181 cggctgctgc tggcgggcgc cgaggtgcag gccaagctgg agaacgccga agtgctggag
      241 ctgacggtgc ggcgggtcca gggtgtgctg cggggccggg cgcgcgagcg cgagcagctg
      301 caggcggaag cgagcgagcg cttcgctgcc ggctacatcc agtgcatgca cgaggtgcac
      361 acgttcgtgt ccacgtgcca ggccatcgac gctaccgtcg ctgccgagct cctgaaccat
      421 ctgctcgagt ccatgccgct gcgtgagggc agcagcttcc aggatctgct gggggacgcc
      481 ctggcggggc cacctagagc ccctggacgg agtggctggc ctgcgggggg cgctccggga
      541 tccccaatac ccagcccccc gggtcctggg gacgacctgt gctccgacct ggaggaggcc
      601 cctgaggctg aactgagtca ggctcctgct gaggggcccg acttggtgcc cgcagccctg
      661 ggcagcctga ccacagccca aattgcccgg agtgtctgga ggccttggtg cccaactttc
      721 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
      781 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      841 tgtggaaagg acgattcgga caggtcccaa gagctagc
//
