LOCUS       sequence-286812-        1670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3423
FEATURES             Location/Qualifiers
     source          1..1670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1502..1526)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1534..1575
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg aaaaccttac aaaacacagc
       61 attgagtgtt caagtttcag aggtgattgg gaatgtaaaa accagtttga gagaaaacag
      121 ggatctcagg aaggacattt cagtgaaatg atatttactc ctgaagacat gcccactttc
      181 agtatccagc atcagagaat tcatactgat gagaaactcc ttgaatgtaa ggaatgtggg
      241 aaggatttta gttttgtatc agtccttgtt cgacatcagc gaattcatac tggtgagaaa
      301 ccttatgaat gcaaagaatg tggcaaggcc tttggtagtg gtgcaaacct tgcttaccat
      361 caaagaattc atactggtga gaagcctttt gaatgtaaag aatgtgggaa ggcctttggt
      421 agtggctcaa accttactca ccatcagaga attcatactg gtgagaaacc ctatgagtgt
      481 aaggaatgtg ggaaagcctt tagttttgga tcaggcctta ttcgacatca gatcattcac
      541 agtggtgaga agccttatga gtgtaaggaa tgtgggaagt cctttagttt tgaatcagcc
      601 cttattcggc atcacagaat tcacacaggt gagaaacctt atgaatgtat agattgtggt
      661 aaagcctttg gcagtggttc aaaccttact caacatcggc ggattcatac tggtgagaaa
      721 ccttatgaat gcaaagcatg tggaatggcc tttagcagtg gttcggctct tactcggcat
      781 cagagaattc ataccggtga gaaaccatat atatgtaatg aatgtggtaa ggcctttagt
      841 tttggatcag cccttactcg acatcaaaga attcatactg gtgagaaacc ttatgtatgt
      901 aaggaatgtg ggaaggcttt taatagtggc tcagatctca ctcagcatca gagaattcac
      961 actggtgaga aaccctatga gtgtaaggag tgtgagaaag cctttagaag tggttcaaaa
     1021 cttattcagc atcaaagaat gcatactgga gagaaacctt atgaatgtaa ggaatgtggg
     1081 aagaccttta gtagtggttc agaccttact caacatcaca gaattcatac tggtgagaaa
     1141 ccctatgaat gtaaggaatg tgggaaggcc tttggtagtg gctcaaaact tatccaacac
     1201 cagctaatcc atactggtga aagaccctat gaatgtaaag aatgtggaaa gtcctttagt
     1261 agtggttcag ctcttaatcg gcaccagaga atacacactg gtgagaaacc ctatgaatgt
     1321 aaggagtgtg ggaaggcttt ttatagtggc tcaagcctta ctcagcatca gagaattcat
     1381 acaggtgaga aactttatga atgtaagaac tgtgggaagg cttatgggag ggattcagag
     1441 tttcagcaac ataagaaaag tcataatggt aagaaactct gcgaattgga aactataaat
     1501 tgcccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     1561 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1621 ctttatatat cttgtggaaa ggacgatgtt aaaattctca gctcttgacc
//
