LOCUS       sequence-286815-        1871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3424
FEATURES             Location/Qualifiers
     source          1..1871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1703..1727)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1735..1776
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg actcagtggt ctttgaggat
       61 gtggctgtgg acttcaccct ggaggagtgg gctttgctgg attctgctca gagggacctc
      121 tacagagatg tgatgctgga gaccttccgg aacctggcct cagtagatga tgggactcaa
      181 tttaaagcca atgggtcagt ttctctgcag gatatgtacg ggcaagaaaa atctaaggaa
      241 cagacaatac caaacttcac aggaaataat tcctgtgcct acactttaga aaaaaattgt
      301 gaaggctatg gcactgaaga ccaccacaaa aatctgagaa atcatatggt ggacagattc
      361 tgtacacata atgaaggtaa tcaatatgga gaagccatcc atcaaatgcc agatcttacc
      421 ctgcacaaga aagtttctgc tggagaaaaa ccatatgaat gcaccaagtg caggacagtc
      481 ttcacgcatc tttcttctct taaaaggcac gtcaagtctc actgtggacg aaaagcacct
      541 ccaggtgagg aatgtaagca ggcctgcatt tgtccctcac acctacacag tcacggaaga
      601 accgacactg aggagaagcc gtataagtgt caagcatgtg ggcaaacttt ccaacatcct
      661 cgttacctct cccaccacgt aaagactcac acagcagaga aaacctacaa atgcgagcag
      721 tgtcggatgg cgtttaatgg gttcgcaagc ttcactagac atgtgagaac tcacacaaaa
      781 gacaggccat ataaatgtca ggaatgtggg agagccttca tttatccctc gacatttcaa
      841 agacacatga caacacacac tggagagaag ccctataaat gtcagcactg tgggaaagcc
      901 ttcacttacc cccaggcttt tcaaagacat gagaagacgc acacgggaga gaagccctat
      961 gaatgcaagc agtgtgggaa aacattcagt tggtctgaaa ccttgcgagt ccacatgagg
     1021 atccacaccg gggacaaact ctataaatgt gaacactgtg ggaaggcttt tacctcttcc
     1081 agatcattcc aaggtcattt gaggacgcac actggagaga aaccttatga gtgtaaacaa
     1141 tgtgggaaag ccttcacttg gtcctcaacg tttagagaac atgtgagaat tcacacgcaa
     1201 gagcagctct ataaatgtga acaatgtggg aaggctttta cctcttccag atcattccga
     1261 ggtcatttga ggacgcacac tggagagaag ccttatgagt gtaaacaatg tggaaaaacc
     1321 ttcacttggt cctcaacgtt tagagaacat gtgagaattc acacgcaaga gcagctccat
     1381 aaatgtgaac actgtgggaa ggcctttacc tcttccagag cattccaagg tcatttgagg
     1441 atgcacactg gagagaagcc ttatgagtgt aaacaatgtg gaaaaacctt cacttggtcc
     1501 tcaaccttac ataatcatgt gaggatgcac actggagaga aacctcacaa atgtaaacaa
     1561 tgtgggatgt ccttcaagtg gcactcctcc ttccggaacc atctgaggat gcacacagga
     1621 cagaaatccc acgaatgtca gtcatactca aaagccttca gttgccaagt cattctttct
     1681 aaaaccagtg agagcacaca ctacccaact ttcttgtaca aagtggttga tatcggtaag
     1741 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1801 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgatgg tagcagcagg
     1861 ccgatatttt a
//
