LOCUS       sequence-286818-        1820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3425
FEATURES             Location/Qualifiers
     source          1..1820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1652..1676)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1684..1725
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg tggctgggtg gttgacaaat
       61 tactctcagg actcagtgac ctttgaggat gtggctgtgg acttcaccca ggaggagtgg
      121 actttgctgg atcaaactca gagaaactta tacagagatg tgatgctgga gaactataag
      181 aatctagttg cagtagattg ggagagtcat attaatacca aatggtcagc acctcagcag
      241 aattttttgc aggggaaaac atccagtgtg gtggaaatgg agagaaacca ttttggagag
      301 gaactgtttg actttaacca atgtgaaaaa gccttgagtg aacactcatg ccttaagact
      361 cacaggagaa cttactttag aaagaaaacc tgtgagtgta atcaatgtga aaaagccttc
      421 agaaaaccct ctatctttac tttacacaag aaaactgata tcggagagga acttcctaac
      481 tgtaatcaat gtgaaacagc cttcagccaa catctacatc ttgtttgcaa gaaaactagc
      541 caaaatctac atcttgtttg caagaaaact cacactcaag agaaaccata taaatgcagt
      601 gactgtgaaa aaggcttacc ttcctcctca cacctcagag aatgtgtaag aatttatggt
      661 ggagagagac catatactca taaggagtat gtcgaaacct tttctcattc tacagccctt
      721 tttgtacaca tgcaaactca agatggagaa aaattctatg aatgtaaagc atgtgggaaa
      781 cccttcactg agtcgtcata tcttactcaa catttaagaa ctcatagtag agtgttacct
      841 atagaacata agaaatttgg caaagccttt gctttttccc cagatcttgc taaacatata
      901 agacttagaa ctagaggaaa acactatgtt tgtaatgaat gtggcaaaga atttacttgt
      961 ttctcaaaac tcaacattca cataagggtt cacactggag aaaaaccgta tgagtgcaac
     1021 aaatgtggga aagccttcac tgattcatca ggtcttataa aacacaggcg aactcacact
     1081 ggagaaaagc cttatgaatg taaggaatgt gggaaagctt ttgctaactc ttcacatctt
     1141 actgtacata tgagaactca cactggtgag aagccttatc aatgtaagga atgtggaaaa
     1201 gcctttatta attcctcttc ctttaaaagt cacatgcaga ctcatcctgg tgtaaaaccc
     1261 tatgactgtc aacagtgtgg gaaagccttc attcgatcct catttcttat tcgacatttg
     1321 agaagtcaca gtgcagaaag gccttttgaa tgtgaggaat gtgggaaagc ctttagatac
     1381 tcctcgcacc ttagtcaaca taaaagaata catacagggg agaggccata taaatgtcaa
     1441 aagtgtgggc aagccttcag tatctcatca ggccttacag tacacatgag aactcacact
     1501 ggtgaacggc cctttgaatg tcaggaatgt gggaaagcct ttactcggtc cacatatctt
     1561 attcgacatc taagaagtca tagtgtggag aaaccatata aggaatgtgg gcaaaccttt
     1621 agtaattcct catgccttac tgaatgtgtg tgcccaactt tcttgtacaa agtggttgat
     1681 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     1741 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgacaaa
     1801 cacctcaacc gattatcgtc
//
