LOCUS       sequence-286821-        1802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3426
FEATURES             Location/Qualifiers
     source          1..1802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1634..1658)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1666..1707
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga tccaggccca ggaatccata
       61 acactggagg atgtggctgt ggacttcact tgggaggagt ggcaactcct gggcgctgct
      121 cagaaggacc tgtaccggga cgtgatgttg gagaactaca gcaacctggt ggcagtgggg
      181 tatcaagcca gcaaaccgga tgcactcttc aagttggaac aaggagaaca actgtggaca
      241 attgaagatg gaatccacag tggagcctgt tcagacatat ggaaagttga tcatgtgctg
      301 gagcgcttgc agagtgaaag cctggtgaac agaaggaaac catgtcatga acatgatgca
      361 tttgaaaata ttgttcattg cagcaaaagt cagtttctgt tagggcaaaa tcatgatata
      421 tttgacttac gtggaaaaag tttgaaatcc aatttaactt tagttaacca gagcaaaggc
      481 tatgaaataa agaactctgt tgagtttact ggaaatgggg actcctttct tcatgctaac
      541 catgaacgac ttcatactgc aattaaattc cctgcaagtc aaaaactcat cagcactaag
      601 tcccaattca tcagtcccaa gcatcagaaa acacgaaaat tagagaagca tcatgtgtgc
      661 agtgaatgtg ggaaagcctt catcaagaag tcttggctaa ctgatcacca ggtaatgcat
      721 acaggagaga aaccccacag atgtagtcta tgtgagaaag ccttctccag aaagttcatg
      781 cttactgaac atcagcgaac tcatacagga gaaaaacctt atgaatgccc tgaatgtggc
      841 aaagcctttc tcaagaaatc acggctcaac atacatcaga aaacacatac cggagagaaa
      901 ccctatatat gcagtgaatg tggaaaaggc ttcatccaga aaggaaatct cattgtacac
      961 cagcgaattc atacaggtga gaaaccttat atatgcaatg aatgtggaaa aggcttcatt
     1021 cagaagacgt gtctcatagc acatcagaga tttcacacag gaaagacgcc ctttgtgtgc
     1081 agtgaatgtg gaaaatcctg ttctcagaaa tcaggtctca ttaaacatca aagaattcac
     1141 acaggagaga aaccctttga atgtagtgaa tgtgggaaag cctttagcac aaagcaaaag
     1201 ctcattgtcc atcaaaggac tcatacagga gagagaccct atggctgtaa cgagtgtggg
     1261 aaagcgtttg cgtatatgtc gtgtctggtt aagcataaga gaatacacac aagggagaaa
     1321 caagaggcag ccaaggtgga aaatcctcct gcagagaggc acagctcatt acacaccagt
     1381 gatgtcatgc aggagaaaaa ctctgctaac ggggcgacta cacaagtgcc ttctgtggcc
     1441 cctcagacat cattaaacat cagcggcctc ctcgcaaaca ggaacgtagt ccttgtggga
     1501 cagccagtgg tcagatgtgc agcctcagga gataacagag gatttgcaca ggacagaaac
     1561 cttgtgaatg cagtgaatgt ggttgtgcct tccgtgatca attatgtctt attttatgtt
     1621 acagaaaacc catacccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     1681 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     1741 tcgatttctt ggctttatat atcttgtgga aaggacgatt ttctgcagac atacctttac
     1801 ca
//
