LOCUS       sequence-286827-        1745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3428
FEATURES             Location/Qualifiers
     source          1..1745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1577..1601)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1609..1650
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cctccgggag tgtggccgag
       61 tgcctgcagc aggagaccac ctgccccgtg tgcctgcagt acttcgcaga gcccatgatg
      121 ctcgactgcg gccataacat ctgttgcgcg tgcctcgccc gctgctgggg cacggcagag
      181 actaacgtgt cgtgcccgca gtgccgggag accttcccgc agaggcacat gcggcccaac
      241 cggcacctgg ccaacgtgac ccaactggta aagcagctgc gcaccgagcg gccgtcgggg
      301 cccggcggcg agatgggcgt gtgcgagaag caccgcgagc ccctgaagct gtactgcgag
      361 gaggaccaga tgcccatctg cgtggtgtgc gaccgctccc gcgagcaccg cggccacagc
      421 gtgctgccgc tcgaggaggc ggtggagggc ttcaaggagc aaatccagaa ccagctcgac
      481 catttaaaaa gagtgaaaga tttaaagaag agacgtcggg cccaggggga acaggcacga
      541 gctgaactct tgagcctaac ccagatggag agggagaaga ttgtttggga gtttgagcag
      601 ctgtatcact ccttaaagga gcatgagtat cgcctcctgg cccgccttga ggagctagac
      661 ttggccatct acaatagcat caatggtgcc atcacccagt tctcttgcaa catctcccac
      721 ctcagcagcc tgatcgctca gctagaagag aagcagcagc agcccaccag ggagctcctg
      781 caggacattg gggacacatt gagcagggct gaaagaatca ggattcctga accttggatc
      841 acacctccag atttgcaaga gaaaatccac atttttgccc aaaaatgtct attcttgacg
      901 gagagtctaa agcagttcac agaaaaaatg cagtcagata tggagaaaat ccaagaatta
      961 agagaggctc agttatactc agtggacgtg actctggacc cagacacggc ctaccccagc
     1021 ctgatcctct ctgataatct gcggcaagtg cggtacagtt acctccaaca ggacctgcct
     1081 gacaaccccg agaggttcaa tctgtttccc tgtgtcttgg gctctccatg cttcatcgcc
     1141 gggagacatt attgggaggt agaggtggga gataaagcca agtggaccat aggtgtctgt
     1201 gaagactcag tgtgcagaaa aggtggagta acctcagccc cccagaatgg attctgggca
     1261 gtgtctttgt ggtatgggaa agaatattgg gctcttacct ccccaatgac tgccctaccc
     1321 ctgcggaccc cgctccagcg ggtggggatt ttcttggact atgatgctgg tgaggtctcc
     1381 ttctacaacg tgacagagag gtgtcacacc ttcactttct ctcatgctac cttttgtggg
     1441 cctgtccggc cctacttcag tctgagttac tcgggaggga aaagtgcagc tcctctgatc
     1501 atctgcccca tgagtgggat agatgggttt tctggccatg ttgggaatca tggtcattcc
     1561 atggagacct ccccttgccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1621 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1681 atttcgattt cttggcttta tatatcttgt ggaaaggacg attaaccagt gagcacccga
     1741 gaagc
//
