LOCUS       sequence-286830-        1403 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3429
FEATURES             Location/Qualifiers
     source          1..1403
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1235..1259)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1267..1308
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt ctcaggtgac atttagtgat
       61 gtggctatag acttctctca tgaagagtgg gcatgcctag attctgctca gagggactta
      121 tacaaggatg tgatggtcca gaattatgag aacctggtct ctgtaggtct ttccgtaact
      181 aagccatatg tgatcatgtt attggaggat ggaaaagagc cctggatgat ggagaaaaaa
      241 ctgtcaaaag attgggaatc aagatgggaa aacaaggaat tatcaacaaa gaaggatatt
      301 tatgatgaag attcacccca accagtaaca atggaaaaag ttgtaaaaca aagttatgaa
      361 ttttcaaatt ctaataagaa tttggaatat acagaatgcg acacatttag aagcaccttt
      421 cattcaaagt ctactctttc tgaaccacaa aacaattctg ctgaagggaa ttcacacaaa
      481 tatgatatat taaagaagaa tttatcaaaa aagtcagtta taaaaagtga gagaataaat
      541 ggtggaaaga aacttttaaa ttctaataaa agtggggcag ccttcaacca gagcaaatct
      601 cttacccttc cccagacttg taatagagag aaaatctata catgcagtga atgtgggaaa
      661 gcctttggca aacagtcaat cctcagtcgc cactggagaa ttcatacagg agagaagccc
      721 tatgaatgtc gtgaatgtgg gaagactttt agccatggtt catcccttac acgacatcag
      781 ataagccata gtggagagaa accttacaaa tgcattgaat gtgggaaggc ctttagccat
      841 ggctcatcac ttactaacca tcagagcact cacacgggag agaaaccgta tgaatgtatg
      901 aactgtggaa agtcttttag tcgtgtgtcc cttctcattc agcatctaag aattcatacg
      961 caagaaaaac gctatgagtg tcgtatatgt ggaaaggcct tcattcatag ttcgtctctc
     1021 attcaccatc agaaaagcca tactggagag aagccttatg aatgtagaga atgtgggaaa
     1081 gctttctgct gtagctcaca ccttactcaa catcaaagaa ttcacagtat gaagaaaaaa
     1141 tatgaatgca acaaatgtct caaggtcttt agtagcttct catttcttgt tcaacatcag
     1201 agtattcata ctgaagaaaa accgtttgaa gtttacccaa ctttcttgta caaagtggtt
     1261 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     1321 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgac
     1381 ttactcgaga agcctaggag atg
//
